Publications

2024 Publications

303. Fluegel, L. L.; Deng, M. -R.; Su, P.; Kalkreuter, E.; Yang, D.; Rudolf, J. R.; Dong, L. -B.; Shen, B. (2024) Development of platensimycin, platencin, and platensilin overproducers by biosynthetic pathway engineering and fermentation medium optimization. J. Ind. Microbiol. Biotechnol. 

302. Xu, Z.F.; Zhou, Y.L.; Bo, S.T.; Zhang, S.Q.; Shi, J.; Xiang, L.; Xi, Y.X.; Zhang, B.; Xu, Z.R.; Yang, D.; Shen, B.; Tan, R.X.; Ge, H.M.; (2024) Discovery and biosynthetic pathway analysis of cyclopentane–β-lactone globilactone A. Nat. Synth. 3:99–110

2023 Publications

301. Gui, C.; Kalkreuter, E.; Liu, YC.; Li, G.; Steele, AD.; Yang, D.; Chang, C.; Shen, B. (2023) Cofactorless oxygenases guide anthraquinone-fused enediyne biosynthesis. Nat. Chem. Biol. 20:243–250

300. Bader, C.D.; Nichols, A.L.; Yang, D.; Shen, B. (2023) Interplay of Emerging and Established Technologies drives Innovation in Natural Product Antibiotic Discovery. Curr. Opin. Microbiol. 75:102359

299. Steele, A.D.; Kiefer, A.F.; Shen, B. (2023) The Many Facets of Sulfur Incorporation in Natural Product Biosynthesis. Curr. Opin. Chem. Biol. 76:102366

298. Bhardwaj, M.; Cui, Z.; Daniel Hankore, E.; Moonschi, F. H.; Saghaeiannejad Esfahani, H.; Kalkreuter, E.; Gui, C.; Yang, D.; Phillips, G. N.; Jr., Thorson, J. S.; Shen, B.; Van Lanen, S. G. (2023) A discrete intermediate for the biosynthesis of both the enediyne core and the anthraquinone moiety of enediyne natural products. Proc. Natl. Acad. Sci. U.S.A. 120(9): e2220468120.

297. Cao, M.; Yang, D.; Adhikari, A.; Ye, F.; Zheng, C.; Yan, W.; Meng, S.; Su, P.; Shen, B. (2023) Neogrisemycin, a Trisulfide-Bridged Angucycline, Produced upon Expressing the Thioangucycline Biosynthetic Gene Cluster in Streptomyces albus J1074. Org. Lett. 25:961–965

296. Vega, V. F.; Yang, D.; Jordán, L. O.; Ye, F.; Conway, L.; Chen, L. Y.; Shumate, J.; Baillargeon, P.; Scampavia, L.; Souza, G.; Parker, C.; Shen, B.; Spicer, T. P. (2023) Protocol for 3D Screening of Lung Cancer Spheroids using Natural Products. SLAS Discov.

295. Steele, A. D.; Kiefer, A. F.; Hwang, D.; Yang, D.; Teijaro, C. N.; Adhikari, A.; Rader, C.; Shen, B.(2023) Application of a Biocatalytic Strategy for the Preparation of Tiancimycin-based Antibody-Drug Conjugates Revealing Key Insights into Structure-Activity Relationships. J. Med. Chem. 66(2):1562–1573.

2022 Publications

294. Gui, C.; Kalkreuter, E.; Liu, Y. C.; Adhikari, A.; Teijaro, C. N.; Yang, D.; Changsoo, C.; Shen, B. (2022) Intramolecular C–C Bond Formation Links Anthraquinone and Enediyne Scaffolds in Tiancimycin Biosynthesis. JACS. 144(44), 20452-20462.

293. Yang, D.; Ye, F; Teijaro, C. N.; Adhikari, A.; Hwang, D..; Annaval, T.; Li, G.; Yan, X.; Gui, C.; Rader, C.; Shen, B. (2022) Functional Characterization of Cytochrome P450 Hydroxylase YpmL in Yangpumicin A Biosynthesis and Its Application for Anthraquinone-Fused Enediyne Structural Diversification. Org. Lett. 24:1219-1223.

292. Ye, F.; Haniff, H., Suresh, B. M.; Yang, D.; Zhang, P.; Crynen, G.; Teijaro, C. N.; Yan, W., Abegg, D.; Adibekian, A.; Shen, B.; Disney, M. D. (2022) Rational Approach to Identify RNA Targets of Natural Products Enables Identification of Nocathiacin as an Inhibitor of an Oncogenic RNA. ACS Chem. Biol. 17:474–482.

291. Kalkreuter, E; Shen, B. (2022) MIO-containing aminomutases for α- to β-amino acids. Trends Chem. 4:91-92.

2021 Publications

290. Meng, S.; Steele, A. D.; Yan, W.; Pan, G.; Kalkreuter, E.; Liu, Y.-C.; Xu, Z.; Shen, B. (2021) Thiocysteine lyases as polyketide synthase domains installing hydropersulfide into natural products and a hydropersulfide methyltransferase. Nat. Commun. 12:5672.

289. Annaval, T.; Teijaro, C. N.; Adhikari, A.; Yan, X.; Chen, J.-J.; Crnovcic, I.; Yang, D.; Shen, B. (2021) Cytochrome P450 hydroxylase TnmL catalyzing sequential hydroxylation with an additional proof-reading reactivity in tiancimycin biosynthesis. ACS Chem. Biol. 16:in press.

288. Hindra; Yang, D.; Luo, J.; Huang, T.; Yan, X.; Adhikari, A.; Teijaro, C. N.; Ge, H.; Shen, B. (2021) Submerged fermentation of Streptomyces uncialis providing a biotechnology platform for uncialamycin biosynthesis, engineering, and production. J. Ind. Microbiol. Biotechnol. 48:kuab025 (Special Issue on Natural Product Discovery and Development in Genomic Era).

287. Luo, J.; Yang, D.; Hindra; Adhikari, A.; Dong, L.-B.; Ye, F.; Yan, X.; Rader, C.; Shen, B. (2021) Discovery of ammosesters by mining the Streptomyces uncialis DCA2648 genome revealing new insight into ammosamide biosynthesis. J. Ind. Microbiol. Biotechnol. 48:kuab027 (Special Issue on Natural Product Discovery and Development in Genomic Era).

286. Shen, B.; Tang, T.; Baltz, R. H.; Ramon Gonzalez, R. (2021) Introduction to the special issue: “Natural Product Discovery and Development in the Genomic Era”. J. Ind. Microbiol. Biotechnol. 48:kuab030.

285. Li, Y.; Weng, X.; Deng, Y.; Pan, J.; Zhu, S.; Wen, Z.; Yuan, Y.; Li, S.; Shen, B.; Duan, Y.; Huang, Y. (2021) Semisynthesis and biological evaluation of platencin thioether derivatives: Dual FabF and FabH inhibitors against MRSA. ACS Med. Chem. Lett. 12:433-442.

284. Cao, M.; Zheng, C.-J.; Yang, D.; Kalkreuter, E.; Adhikari, A.; Liu, Y.-C.; Rateb, M. E.; Shen, B. (2021) Cryptic sulfur incorporation in thioangucycline biosynthesis. Angew. Chem. Intl. Eng. 60:7140-7147.

283. Adhikari, A.; Shen, B.; Rader, C. (2021) Challenges and opportunities to develop enediyne natural products as payloads for antibody-drug conjugates. Antib. Ther. 4:1-15.

2020 Publications

282. Zheng, C.-J.; Kalkreuter, E.; Fan, B.-Y.; Liu, Y.-C.; Dong, L.-B.; Shen, B. (2020) PtmC catalyzes the final step of thioplatensimycin, thioplatencin, and thioplatensilin biosynthesis and expands the scope of arylamine N-acetyltransferases. ACS Chem. Biol. 16:96-105. (https://doi.org/10.1021/acschembio.0c00773)

281. Lohman, J. R.; Shen, B. (2020) The LnmK bifunctional acyltransferase/decarboxylase specifying (2R)-methylmalonyl-CoA and employing substrate-assisted catalysis for polyketide biosynthesis. Biochemistry 59:4143-4147. (https://doi.org/10.1021/acs.biochem.0c00749)

280. Zhang, X.; King-Smith, E.; Dong, L.-B., Yang, L.-C., Rudolf, J. D.; Shen, B.; Renata, H. (2020) Divergent synthesis of complex diterpenes through a hybrid oxidative approach. Science 368:799-806. (https://doi.org/10.1126/science.abb8271)

279. Adhikari, A.; Teijaro, C. N.; Yan, X., Chang, C.-Y., Gui, C., Liu, Y.-C., Crnovcic, I., Yang, D., Annaval, T., Rader, C., Shen, B. Characterization of TnmH as an O-methyltransferase revealing in-sights into tiancimycin biosynthesis and enabling a biocatalytic strategy to prepare antibody-tiancimycin conjugates. J. Med. Chem. 2020, 63:8432-8441. (https://doi.org/10.1021/acs.jmedchem.0c00799)

278. Wang, Z.; Liu, X.; Peng, Y.; Su, M.; Zhu, S.; Pan, J.; Shen, B.; Duan, Y.; Fuang, Y. (2020) Platensimycin-encapsulated liposomes or micelles as biosafe nanoantibiotics exhibited strong antibacterial activities against methicilin-resistant Staphylococcus aureus infection in mice. Mol. Pharm. 17:2451-2462. (https://doi.org/10.1021/acs.molpharmaceut.0c00194)

277. Liu, X.; Wang, Z.; Feng, X.; Bai, E.; Xiong, Y.; Zhu, X.; Shen, B.; Duan, Y.; Huang, Y. (2020) Platensimycin-encapsulated poly(lactic-co-glycolic acid) and poly(amidoamine) dendrimers nanoparticles with enhanced anti-staphylococcal activity in vivo. Bioconj. Chem. 31:1425-1437. (https://doi.org/10.1021/acs.bioconjchem.0c00121)

276. Zhou, W.; Liang, H.; Qin, X.; Cao, D.; Zhu, X.; Ju, J.; Shen, B.; Duan, Y.; Huang, Y. (2020) The isolation of pyrroloformamide congeners and characterization of their biosynthetic gene cluster. J. Nat. Prod. 83:202-209. (https://doi.org/10.1021/acs.jnatprod.9b00321)

275. Kalkreuter, E.; Pan, G.; Cepeda, A. J.; Shen, B. (2020) Targeting bacterial genomes for natural product discovery: opportunities, challenges, and strategies. Trends Pharmacol. Sci. 41:13-26 (https://doi.org/10.1016/j.tips.2019.11.002)

274. Steele, A.; Kalkreuter, E.; Pan, G.; Meng, S.; Shen, B. (2019) “Hybrid Peptide-Polyketide Natural Product Biosynthesis” in in Liu, H.-W.; Begley, T. P. (eds.) Comprehensive Natural Products III: Chemistry and Biology, Vol 2, 284-335. Elsevier. (https://doi.org/10.1016/B978-0-12-409547-2.14651-7)

273. Adhikari, A.; Teijaro, C. N.; Townsend, C. A.; Shen, B. (2020) “Biosynthesis of Enediyne Natural Products” in Liu, H.-W.; Begley, T. P. (eds.) Comprehensive Natural Products III: Chemistry and Biology, Vol 1, 365-414. Elsevier. (https://doi.org/10.1016/B978-0-12-409547-2.14669-4)

2019 Publications

272. Steele, A. D.; Teijaro, C. N.; Yang, D.; Shen, B. (2019) Leveraging a large microbial strain collection for natural product discovery. J. Biol. Chem. 294:16567-16576 (https://doi.org/10.1074/jbc.REV119.006514)

271. Wang, Z.; Wen, Z.; Liu, L.; Zhu, X.; Shen, B.; Yan, X.; Duan, Y.; Huang, Y. (2019) Yangpumicins F and G, enediyne congeners from Micromonospora yangpuensis DSM 45577. J. Nat. Prod. 82:2483-2488 (https://doi.org/10.1021/acs.jnatprod.9b00229)

270. Liang, H.; Jiang, L.; Jiang, Q.; Shi,J.; Xiang, J.; Yan, X.; Zhu, X.; Zhao, L.; Shen, B.; Duan, Y.; Huang, Y. (2019) A 3-hydroxy-3-methylglutaryl-CoA synthase-based probe for the discovery of the acyltransferase-less type I polyketide synthases. Environ Microbiol. 21:4270-4282 (https://doi.org/10.1111/1462-2920.14787)

269. Dong, L.-B.; Liu, Y.-C.; Cepeda, A. J.; Kalkreuter, E.; Deng, M.-R.; Rudolf, J. D.; Chang, C.; Joachimiak, A.; Phillips Jr, G. N.; Shen, B. (2019) Characterization and crystal structure of a nonheme diiron monooxygenase involved in platensimycin and platencin biosynthesis. J. Am. Chem. Soc. 141:12406-12412 (https://doi.org/10.1021/jacs.9b06183)

268. Deng, Y.; Weng, X.; Li, Y.; Su, M.; Wen, Z.; Ji, X.; Ren, N.; Shen, B.; Duan, Y.; Huang, Y. (2019) Late-stage functionalization of platensimycin leading to multiple analogues with improved antibacterial activity in vitro and in vivo. J. Med. Chem. 62:6682-6693 (https://doi.org/10.1021/acs.jmedchem.9b00616)

267. Su, M.; Qiu, L.; Deng, Y.; Ruiz, C.; Rudolf, J. D.; Dong, L.-B.; Feng, X.; Cameron, M. D.; Shen, B.; Duan, Y.; Huang, Y. (2019) Evaluation of platensimycin and platensimycin-inspired thioether analogues against methicillin-resistant Staphylococcus aureus in topical and systemic infetion mouse models. Mol. Pharmaceutics 16:3065-3071 (https://doi.org/10.1021/acs.molpharmaceut.9b00293)

266. Xu, Z.; Fang, S.M.; Bakowski, M. A.; Rateb, M. E.; Yang, D.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Duan, Y.; Hull, M.; McNamara, C. W.; Shen, B. (2019) Discovery of Kirromycins with Anti-Wolbachia Activity from Streptomyces sp. CB00686. ACS Chem. Biol. 14: 1174-1182 (https://pubs.acs.org/doi/10.1021/acschembio.9b00086)

265. Qiu, L.; Wen, Z.; Li, Y.; Tian, K.; Deng, Y.; Shen, B.; Duan, Y.; Huang, Y. (2019) Stereoselective functionalization of platensimycin and platencin by Sulfa-Michael/Aldol reactions. Org. Biomol. Chem. 17:4261-4272 (https://doi.org/ 10.1039/C9OB00324J)

264. Dong, L.-B.; Zhang, X.; Rudolf, J. D.; Deng, M.-R.; Kalkreuter, E.; Cepeda, A. J.; Renata, H.; Shen, B. (2019) Cryptic and stereoselective hydroxylation, oxidation, and reduction in platensimycin and platencin biosynthesis. J. Am. Chem. Soc. 141:4043-4050 (https://doi.org/10.1021/jacs.8b13452)

263. Teijaro, C. N.; Adhikari, A.; Shen, B. (2019) Challenges and opportunities in microbial engineering for natural products. J. Ind. Microbiol. Biotechnol. 46:433-444 (https://doi.org/10.1007/s10295-018-2094-5). (SI on Natural Products Discovery and Development)

262. McApline, J.; et al.; Pauli, G. (2018) The value of universally available raw NMR data for transparency, reproducibility, and integrity in natural product research. Nat. Prod. Rep. 36:35-107 (https://doi.org/10.1039/c7np00064b)

2018 Publications

261. Tian, K.; Deng, Y.; Qiu, L.; Zhu, X.; Shen, B.; Duan, Y.; Huang, Y. (2018) Semisynthesis and biological evaluation of platensimycin analogues with varying aminiobenzoic acids. ChemistrySelect, 3:12625-12629 (https://doi.org/10.1002/slct.201802475).

260. Deng, Y.; Su, M.; Kang, D.; Liu, X.; Wen, Z.; Li, Y.; Qiu, L.; Shen, B.; Duan, Y.; Huang, Y. (2018) Semisynthesis of platensimycin derivatives with antibiotic activities in mice via Suzuki-Miyaura cross-coupling reactions. J. Med. Chem. 61:11341-11348 (https://pubs.acs.org/doi/10.1021/acs.jmedchem.8b01580).

259. Xu, Z.; Pan, G.; Zhou, H.; Shen, B. (2018) Discovery and characterization of 1-aminocyclopropane-1-carboxylate synthase of bacterial origin. J. Am. Chem. Soc. 140:16957-16961 (https://pubs.acs.org/doi/10.1021/jacs.8b11463).

258. Kearney, S. E.; Zahoranszky-Kohalmi, G.; et al.; Guha, R.; Rohde, J. M. (2018) Canvass: a crowd-sourced, natural product screening library for exploring biological space. ACS Cent. Sci. 4:1727-1741 (https://pubs.acs.org/doi/10.1021/acscentsci.8b00747).

257. Rudolf, J. D.; Dong, L.-B.; Zhang, X.; Renata, H.; Shen, B. (2018) Cytochrome P450-catalyzed hydroxylation initiating ether formation in platensimycin biosynthesis. J. Am. Chem. Soc. 140:12349-12353 (https://doi.org/10.1021/jacs.8b08012).

256. Yan, X.; Chen, J.-J.; Adhikari, A.; Teijaro, C.; Ge, H.; Crnovcic, I.; Chang, C.-Y.; Annaval, T.; Yang, D.; Rader, C.; Shen, B. (2018) Comparative studies of the biosynthetic gene clusters for anthraquinone-fused enediynes reveal the tailoring steps of tiancimycin biosynthesis. Org. Lett. 20:5918-5921 (https://doi.org/10.1021/acs.orglett.8b02584).

255. Annaval, T.; Han, L.; Rudolf, J. D.; Xie, G.; Yang, D.; Chang, C.-Y.; Ma. M, Crnovcic, I.; Miller, M. D.; Soman, J.; Xu, W.; Phillips, G. N. Jr.; Shen, B. (2018) Biochemical and structural characterization of TtnD, a prenylated FMN-dependent decarboxylase from the tautomycetin biosynthetic pathway. ACS Chem. Biol. 13:2728-2738 (https://doi.org/10.1021/acschemcio.8b00673).

254. Kwong, T.; Ma, M.; Pan, G.; Hindra; Yang, D.; Yang, C.; Lohman, J. R.; Rudolf, J. D.; Cleveland, J. L.; Shen, B. (2018) P450-catalyzed tailoring steps in leinamycin biosynthesis featuring regio- and stereoselective hydroxylations and substrate promiscuities. Biochemistry, 57:5005-5013 (https://doi.org/10.1021/acs.biochem.8b00623).

253. Murray, V.; Chen, J. K.; Yang, D.; Shen, B. (2018) The genome-wide DNA sequence specificity of bleomycin analogues in human cells. Bioorg. Med. Chem. 26:4168-4178 (https://doi.org/10.1016/j.bmc.2018.07.006).

252. Jiang, L.; Pu, H.; Xiang, J.; Su, M.; Yan, X.; Yang, D.; Zhu, X.; Shen, B.; Duan, Y.; Huang, Y. (2018) Huanglongmycin A-C, Cytotoxic Nonaketides from Streptomyces sp. CB09001. Front. Chem. 6:article 254 (https://doi.org/10.3389/fchem.2018.00254).

251. Kong, J.; Yi, L.; Xiong, Y.; Huang, Y.; Yang, D.; Yan, X.; Shen, B.; Duan, Y; Zhu, X. (2018) The discovery and development of microbial bleomycin analogues. Appl. Mocribiol. Biotechnol. 102:6791-6798 (https://doi.org/10.1007/s00253-018-9129-8).

250. Dong, L.-B.; Rudolf, J. D.; Deng, M.-R.; Yan, X.; Shen, B. (2018) Discovery of the Tiancilactone Antibiotics by Genome Mining of Atypical Bacterial Type II Diterpene Synthases. ChemBioChem 19:1727-1733 (https://doi.org/10.1002/cbic.201800285)

249. Dong, L.-L.; Rudolf, J. D.; Kang, D.; Wang, N.; He, C. Q.; Deng, Y.; Huang, Y.; Houk, K. N.; Duan, Y.; Shen, B. (2018) Biosynthesis of thiocarboxylic acid-containing natural products. Nat. Commun. 9:2362 (https://doi.org/10.1038/s41467-018-04747).

248. Chang, C.-Y.; Yan, X.; Crnovcic, I.; Annaval, T.; Chang, C.; Nocek, B.; Rudolf, J. D.; Yang, D.; Hindra; Babnigg, G.; Joachimiak, A.; Phillips, G. N. Jr.; Shen, B. (2018) Resistance to enediyne antitumor antibiotics by sequestration. Cell Chem. Biol. 25:1075-1085 (https://doi.org/10.1016/j.chembiol.2018.05.012).

247. Crnovcic, I.; Gan, F.; Yang, D.; Dong, L.-B.; Schultz, P. G.; Shen, B. (2018) Enzymatic characterization of human bleomycin hydrolase and its effect on engineered bleomycin analogues highlighting its potential use as a protein therapeutic against bleomycin induced pulmonary fibrosis. Bioorg. Med. Chem. Lett. 28:2670-2674 (https://doi.org/10.1016/j.bmcl.2018.04.065).

246. Wang, N.; Rudolf, J. D.; Dong, L.-B.; Osipiuk, J.; Hatzos-Skintges, C.; Endres, M.; Chang, C.-Y.; Babnigg, G.; Joachimiak, A.; Phillips, G. N. Jr.; Shen, B. (2018) Natural separation of the acyl-CoA ligase reaction results in a non-adenylating enzyme. Nat. Chem. Biol. 14:730-737 (https://doi.org/10.1038/s41589-018-0061-0).

245. Chang, C.-Y.; Lohman, J. R.; Huang, T.; Michalska, K.; Bigelow, L.; Rudolf, J. D.; Jedrzejczak, R.; Yan, X.; Ma, M.; Babnigg, G.; Joachimiak, A.; Phillips, G. N. Jr.; Shen, B. (2018) Structural insights into the free-standing condensation enzyme SgcC5 catalyzing ester bond formation in biosynthesis of the enediyne antitumor antibiotic C-1027. Biochemistry, 57:3278-3288 (https://doi.org/10.1021/acs.biochem.8b00174).

244. Deng, Y.; Kang, D.; Shi, J.; Zhou, W.; Sun, A.; Ju, J.; Zhu, X.; Shen, B.; Duan, Y.; Huang, Y. (2018) The semi-synthesis, biological evaluation and docking analysis of the oxime, hydrazine and hydrazide derivatives of platensimycin. MedChemComm, 9:789-794 (https://doi.org/10.1039/C8MD00081F).

243. Chen, J.-J.; Rateb, M. E.; Love, M. S.; Xu, Z.; Yang, D.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Duan, Y.; McNamara, C. W.; Shen, B. (2018) Herbicidins from Streptomyces sp. CB01388 showing anti-cryptosporidium activity. J. Nat. Prod. 81:791-797 (https://doi.org/10.1021/acs.jnatprod.7b00850).

242. Qiu, L.; Tian, K.; Wen Z.; Deng, Y.; Kang, D.; Liang, H.; Zhu, X.; Shen, B.; Duan, Y.; Huang, Y. (2018) Biomimetic stereoselective sulfa-Michael addition leads to potent platensimycin and platencin sulfur analogues against methicillin-resistant Staphylococcus aureus. J. Nat. Prod. 81:316-322 (https://doi.org/10.1021/acs.jnatprod.7b00745).

241. Guo, X.; Crnovcic, I.; Chang, C.-Y.; Lohman, J. R.; Papinski, M.; Horsman, G. P.; Bechthold, A.; Shen, B. (2018) PokMT1 from the polyketomycin biosynthetic machinery of Streptomyces diastatochromogenes Tü6028 belongs to the emerging family of C-methyltransferases that act on CoA-activated aromatic substrates. Biochemistry 57:1003-1011 (https://doi.org/10.1021/acs.biochem.7b01219).

240. Liu, L.; Pan, J.; Wang, Z.; Yan, X.; Yang, D.; Zhu, X.; Shen, B.; Duan, Y.; Huang, Y. (2018) Ribosome engineering and fermentation optimization leads to overproduction of tiancimycin A, a new enediyne natural product from Streptomyces sp. CB03234. J. Ind. Microbiol. Biotechnol. 45:141-145 (https://doi.org/10.1007/s10295-018-2014-8).

239. Yan, X.; Hindra; Ge, H.; Yang, D.; Huang, T.; Crnovcic, I.; Chang, C.-Y.; Fang, S.; Annaval, T.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Duan, Y.; Shen, B. (2018) Discovery of alternative producers of the enediyne antitumor antibiotic C-1027 with high titers. J. Nat. Prod. 81:594-599 (https://doi.org/10.1021/acs.jnatprod.7b01013).

238. Zhu, X.; Kong, J.; Yang, H.; Huang, R.; Huang, Y; Yang, D.; Shen, B.; Duan, Y. (2018) Strain improvement by combined UV mutagenesis and ribosome engineering and subsequent fermentation optimization for enhanced 6′-deoxy-bleomycin Z production. Appl. Microbiol. Biotechnol. 102:1651-1661 (https://doi.org/10.1021/acs.jnatprod.7b01013).

237. Yang, D.; Hindra, Dong, L.-B.; Crnovcic, I.; Shen, B. (2018) Engineered production and evaluation of 6′-deoxy-tallysomycin H-1 revealing new insights into the structure-activity relationship of the anticancer drug bleomycin. J. Antibiot. 71:97-103 (https://doi.org/10.1038/ja.2017.93).

236. Nejad, M. I.; Yang, D.; Shen, B.; Gates, K. S. (2018) Oxidative activation of leinamycin E1 triggers alkylation of guanine residues in double-stranded DNA. Chem. Comm. 54:256-259 (https://doi.org/10.1039/C7CC08482J).

2017 Publications

235. Pan, G.; Xu, Z.; Guo, Z.; Hindra; Ma, M.; Yang, D.; Zhou, H.; Gansemans, Y.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Cheng, J.; Van Nieuwerburgh, F.; Suh, J.-W.; Duan, Y.; Shen, B. (2017) Discovery of the leinamycin family of natural products by mining actinobacterial genomes. Proc. Natl. Acad. Sci. USA 114:E11131-E11140 (https://doi.org/10.1073/pnas.1716245115).

234. Yan, X.; Chen, J.-J.; Adhikari, A.; Yang, D.; Crnovcic, I.; Wang, N.; Chang, C.-Y.; Rader, C.; Shen, B. (2017) Genome mining of Micromonospora yangpuensis DSM 45577 as a producer of an anthraquinone-fused enediyne. Org. Lett. 19:6192-6195 (https://doi.org/10.1021/acs.orglett.7b03120).

233. Annaval, T.; Rudolf, J. D.; Chang, C.-Y.; Lohman, J. R.; Kim, Y.; Bigelow, L.; Jedrzejczak, R.; Babnigg, G.; Joachimiak, A.; Phillips, G. N. Jr.; Shen, B. (2017) Crystal structure of the thioesterase SgcE10 supporting common polyene intermediates in 9- and 10-membered enediyne core biosynthesis. ACS Omega 2:5159-5169 (https://doi.org/10.1021/acsomega.7b00933).

232. Rudolf, J. D.; Chang, C.-Y.; Ma, M.; Shen, B. (2017) Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function. Nat. Prod. Rep. 34:1141-1172 (https://doi.org/10.1039/c7np00034k).

231. Zhang, B.; Xu, Z.; Teng, Q.; Pan, G.; Ma, M.; Shen, B. (2017) A Long-range acting dehydratase domain as the missing link for the C17-dehydration in iso-migrastatin biosynthesis. Angew Chem. Int. Ed. 56:7247-7251 ()https://doi.org/10.1002/anie.201703588.

230. Falzone, M.; Crespo, E.; Jones, K.; Khan, G.; Korn, V.; Patel, A.; Patel, M.; Patel, K.; Perkins, C.; Siddiqui, S.; Stenger, A.; Yu, E.; Gelber, M.; Scheffler, R.; Nayda, V.; Ravin, A.; Komal, R.; Rudolf, J.; Shen, B.; Gullo, V.; Demain, A. (2017) Nutritional control of antibiotic production by Streptomyces platensis MA7327: Importance of L-aspartic acid. J. Antibiot. 70:828-831 (https://doi.org/10.1038/ja.2017.49).

229. Hindra; Yang, D.; Teng, Q.; Dong, L.-B.; Crnovcic, I.; Huang, T.; Ge, H.; Shen, B. (2017) Genome mining of Streptomyces mobaraensis DSM40847 as a bleomycin producer providing a biotechnology platform to engineer designer bleomycin analogues. Org. Lett. 19:1386-1389 (https://doi.org/10.1021/acs.orglett.7b00283).

228. Dong, L.-B.; Rudolf, J. D.; Lin, L.; Ruiz, C.; Cameron, M. D.; Shen, B. (2017) In vivo instability of platensimycin and platencin: Synthesis and biological evaluation of urea- and carbamate-platensimycin. Bioorg. Med. Chem. 25:1990-1996 (https://doi.org/10.1016/j.bmc.2017.02.028).

227. Chen, J. K.; Yang, D.; Shen, B.; Murray, V. (2017) Bleomycin analogues preferentially cleave at the transcription start sites of actively transcribed genes in human cells. Int. J. Biochem. Cell Biol. 85:56-65 (https://doi.org/10.1016/j.biocel.2017.02.001).

226. Yang, D.; Rateb, M. E.; Wang, N.; Shen, B. (2017) Competition and co-regulation of spirotoamide and tautomycetin biosynthesis in Streptomyces griseochromogenes and isolation and structural elucidation of spirotoamide C and D. J. Antibiot. 70:710-740 (https://doi.org/10.1038/ja.2017.13).

225. Rudolf, J. D.; Crnovcic, I.; Shen, B. (2017) “Chapter 3, The Role of Combinatorial Biosynthesis in Natural Products Discovery” in Chemical Biology of Natural Products, Cragg, G.; Newman, D.; Grothaus, P. Eds, Taylor and Francis, pp. 87-125 (ISBN 9781439841938).

224. Rudolf, J. D.; Dong, L.-B.; Shen, B. (2017) Platensimycin and platencin: inspiration for chemistry, biology, enzymology, and medicine. Biochem. Pharmacol. 133:139-151 (https://doi.org/10.1016/j.bcp.2016.11.013).

223. Guo, Z.; Pan, G.; Xu, Z.; Yang, D.; Hindra, Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Duan, Y.; Shen, B. (2017) New isofuranonaphthoquinones and isoindolequinones from Streptomyces sp. CB01883. J. Antibiot. 70:414-422 (https://doi.org/10.1038/ja.2016.122).

222. Ma, M.; Rateb, M.; Yang, D.; Rudolf, J.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Duan, Y.; Shen, B. (2017) Germicidins H-J from Streptomyces sp. CB00361. J. Antibiot. 70:200-203 (https://doi.org/10.1038/ja.2016.100).

221. Qiu, L.; Tian, K.; Pan, J.; Jiang, L.; Yang, H.; Zhu, X.; Shen, B.; Duan, Y; Huang, Y. (2017) A facile semi-synthetic approach towards halogen-substituted aminobenzoic acid analogues of platensimycin. Tetrahedron, 73:771-775 (https://doi.org/10.1016/j.tet.2016.12.059).

2016 Publications

220. Rudolf, J. D.; Dong, L.-B.; Shen, B. (2016) Biosynthetic Origin of the Ether Ring in Platensimycin J. Am. Chem. Soc. 138:16711-16721 (https://doi.org/10.1021/jacs.6b09818).

219. Yan, X.; Ge, H.; Huang, T.; Hindra; Yang, D.; Teng, Q.; Crnovcic, I.; Li, X.; Rudolf, J. D.; Lohman, J. R.; Gansemans, Y.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Van Nieuwerburgh, F.; Rader, C.; Duan, Y.; Shen, B. (2016) Strain prioritization and genome mining for enediyne natural products. mBio. 7:e2104-16 (https://doi.org/10.1128/mBio.02104-16).

218. Chen, J. K.; Yang, D.; Shen, B.; Neilan, B. A.; Murry, V. (2016) Zorbamycin has a different DNA sequence selectivity compared with bleomycin and analogues. Bioorg. Med. Chem. 24:6094-6101 (https://doi.org/10.1016/j.bmc.2016.09.072).

217. Huang, Y.; Yang, D.; Pan, G.; Tang, G.-L.; Shen, B. (2016) Characterization of LnmO as a pathway-specific Crp/Fnr-type positive regulator for leinamycin biosynthesis in Streptomyces atroolivaceus and its application for titer improvement. Appl. Microbiol. Biotechnol. 100:10555-10562 (https://doi.org/10.1007/s00253-016-7864-2).

216. Dong, L.; Rudolf, J. D.; Shen, B. (2016) A mutasynthetic library of platensimycin and platencin analogues. Org. Lett. 18:4606-4609 (https://doi.org/10.1021/acs.orglett.6b02248).

215. Chang, C.-Y.; Lohman, J. R.; Cao, H.; Tan, K.; Rudolf, J. D.; Ma, M.; Xu, W.; Bingman, C. A.; Ragothaman, M.; Bigelow, L.; Babnigg, G.; Yan, X.; Joachimiak, A.; Phillips, Jr., G. A.; Shen, B. (2016) Crystal structures of SgcE6 and SgcC, the two-component monooxygenase that catalyzes hydroxylation of a carrier protein-tethered substrate in biosynthesis of the enediyne antitumor antibiotic C-1027 in Streptomyces globisporus. Biochemistry 55:5142-5154 (https://doi.org/10.1021/acs.biochem.6b00713).

214. Huang, Y.; Tang, G.-L.; Pan, G.; Chang, C.-Y.; Shen, B. (2016) Characterization of the ketosynthase and acyl carrier protein domains at the LnmI nonribosomal peptide synthetase-polyketide synthase interface for leinamycin biosynthesis. Org. Lett.18:4288-4291 (https://doi.org/10.1021/acs.orglett.6b02033).

213. Rudolf, J. D.; Dong, L.-B.; Cao, H.; Hatzos-Skintges, C.; Osipiuk, J.; Endres, M.; Chang, C.-Y.; Ma, M.; Babnigg, G.; Joachimiak, A.; Phillips, Jr., G. N.; Shen, B. (2016) Structure of the ent-copalyl diphosphate synthase PtmT2 from Streptomyces platensis CB00739, a bacterial type II diterpene synthase. J. Am. Chem. Soc. 138:10905-10915 (https://doi.org/10.1021/jacs.6b04317).

212. Huang, T.; Chang, C.-Y.; Lohman, J. R.; Rudolf, J. D.; Kim, Y.; Chang, C.; Yang, D.; Ma, M.; Yan, X.; Crnovcic, I.; Bigelow, L.; Clancy, S.; Bingman, C. A.; Yennamalli, R. M.; Babnigg, G.; Joachimiak, A.; Phillips Jr., J. N.; Shen, B. (2016) Crystal structure of SgcJ, an NTF2-like superfamily protein involved in biosynthesis of the 9-membered enediyne antitumor antibiotic C-1027, J. Antibiot. 69:731-740 (https://doi.org/10.1038/ja.2016.88).

211. Dong, L.-B.; Rudolf, J. R.; Shen, B. (2016) Antibacterial sulfur-containing platensimycin and platencin congeners from Streptomyces platensis SB12029. Bioorg. Med. Chem. 24:6348-6353 (https://doi.org/10.1016/j.bmc.2016.04.026).

210. Li, W.; Li, X.; Huang, T.; Teng, Q.; Crnovcic, I.; Rader, C.; Shen, B. (2016) Engineered production of cancer targeting peptide (CTP)-containing C-1027 in Streptomyces globisporus and biological evaluation. Bioorg. Med. Chem. 24:3884-3892. [Pubmed]

209. Shi, J.; Pan, J.; Liu, L.; Yang, D.; Lu, S.; Zhu, X.; Shen, B.; Duan, Y.; Huang, Y. (2016) Titer improvement of pilot-scale production of platensimycin from Streptomyces platensis SB12026. J. Ind. Microbiol. Biotechnol. 43:1027-1035. [Pubmed]

208. Zhang, B.; Yang, D.; Yan, Y.; Pan, G.; Shen, B. (2016) Overproduction of lactimidomycin by cross-overexpression of genes encoding Streptomyces antibiotic regulatory proteins. Appl. Microbiol. Biotechnol. 100:2267-2277. [Pubmed]

207. Smanski, M. J.; Zhou, H.; Claesen, J.; Shen, B.; Fischbach, M. A.; Voigt, C. A. (2016) Synthetic biology to access and expand nature’s chemical diversity. Nat. Rev. Microbiol. 14:135-149. [Pubmed]

206. Rudolf, J. D.; Yan, X.; Shen, B. (2016) Genome neighborhood network reveals insights into enediyne biosynthesis and facilitates prediction and prioritization for discovery. J. Ind. Microbiol. Biotechnol. 42:261-276. [Pubmed]

2015 Publications

205. Shen, B. (2015) A New Golden Age of natural products drug discovery. Cell. 163:1297-1300. [Pubmed]

204. Rudolf, J. D.; Bigelow, L.; Chang, C.; Cuff, M. E.; Lohman, J.R.; Chang, C.-Y.; Ma, M.; Yang, D.; Clancy, S.; Babnigg, G.; Joachimiak, A.; Phillips, G.N. Jr.; Shen, B. (2015) Crystal structure of the zorbamycin-binding protein ZbmA, the primary self-resistance element in Streptomyces flavoviridis ATCC21892. Biochemistry 54:6842-6851. [Pubmed]

203. Lohman, J.R.; Ma, M.; Osipiuk, J.; Nocek, B.; Kim, Y.; Chang, C.; Cuff, M.; Mack, J.; Bigelow, L.; Li, H.; Endres, M.; Babnigg, G.; Joachimiak, A.; Phillips, G.N. Jr.; Shen, B. (2015) Structural and evolutionary relationships of “AT-less” type I polyketide synthase ketosynthases. Proc. Natl. Acad. Sci. U.S.A. 112:12693-12698. [Pubmed]

202. Ma, M.; Rateb, M.; Teng, Q.; Yang, D.; Rudolf, J.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Li, X.; Rader, C.; Duan, Y.; Shen, B. (2015) Angucyclines and angucyclinones from Streptomyces sp. CB01913 featuring C-ring cleavage and expansion. J. Nat. Prod. 78:2471-2480. [Pubmed]

201. Medema, M. H.; et al.; Glockner, F. O. (2015) Minimum information about a biosynthetic gene cluster. Nat. Chem. Biol. 11:625-631. [Pubmed]

200. Ma, M.; Lohman, J. R.; Liu, T.; Shen, B. (2015) C-S bond cleavage by a polyketide synthase domain. Proc. Natl. Acad. Sci. U.S.A. 112:10359-10364. [Pubmed]

199. Rudolf, J. D.; Dong, L.-B.; Huang, T.; Shen, B. (2015) A genetically amenable platensimycin- and platencin-overproducer as a platform for biosynthetic explorations: a showcase of PtmO4, a long-chain acyl-CoA dehydrogenase. Mol. BioSyst. 11:2717-2726. [Pubmed]

198. Liu, T.; Ma, M.; Ge, H.-M.; Yang, C.; Cleveland, J.; Shen, B. (2015) Synthesis and evaluation of 8,4′-dideshydroxy-leinamycin revealing new insights into the structure-activity relationship of the anticancer natural product leinamycin. Bioorg. Med. Chem. Lett. 25:4899-4902. [Pubmed]

197. Huang, S.-X.; Yun, B.-S.; Ma, M.; Basu, H. S.; Church, D. R.; Ingenhorst, G.; Huang, Y.; Yang, D.; Lohman, J. R.; Tang, G.-L.; Ju, J.; Liu, T.; Wilding, G.; Shen, B. (2015) Leinamycin E1 acting as an anticancer prodrug activated by reactive oxygen species. Proc. Natl. Acad. Sci. U.S.A. 112:8278-8283. [Pubmed]

196. Yang, Z.; Cao, S.; Martens, C. A.; Xie, Z.; Ma, M.; Shen, B.; Moss, B (2015) Deciphering poxvirus gene expression by RNA sequencing and ribosome profiling. J. Virology 89:6874-6886. [Pubmed]

195. Rateb, M. E.; Yang, D.; Vodanovic-Jankovic, S.; Yu, Z.; Kron, M. A.; Shen, B. (2015) Adipostatins A-D from Streptomyces sp. 4875 inhibiting Brugia malayi asparaginyl-tRNA synthetase and killing adult Brugia malayi parasites. J. Antibiot. 68:540-542. [Pubmed]

194. Gao, X.; Wan, J.; Liu, B.; Ma, M.; Shen, B.; Qian, S.-B. (2015) Quantitative profiling of initiating ribosomes in vivo. Nat. Methods 12, 147-153. [Pubmed]

193. Shen, B.; Hindra; Yan, X.; Huang, T.; Ge, H.; Yang, D.; Teng, Q.; Rudolf, J. D.; Lohman, J. R. (2015) Enediynes: exploration of microbial genomics to discover new anticancer drug leads. Bioorg. Med. Chem. Lett. 25:9-15. [Pubmed]

2014 Publications

192. Seo, J.-W.; Ma, M.; Kwong, T.; Ju, J.; Lim, S.-K.; Jiang, H.; Lohman, J. R.; Yang, C.; Cleveland, J.; Zazopoulos, E.; Farnet, C. M.; Shen, B. (2014) Comparative characterization of the lactimidomycin and iso-migrastatin biosynthetic machineries revealing unusual features for acyltransferase-less type I polyketide synthases and providing an opportunity to engineer new analogues. Biochemistry 53:7854-7865. [Pubmed]

191. Coughlin, J.; Rudolf, J. D.; Wendt-Pienkowski, E.; Wang, L.; Unsin, C.; Yang, D.; Tao, M.; Shen, B. (2014) BlmB and TlmB provide resistance to the bleomycin family of antitumor antibiotics by N-acetylating metal-free bleomycin, tallysomycin, phleomycin, and zorbamycin. Biochemistry, 53:6901-6909. [Pubmed]

190. Hindra; Huang, T.; Yang, D.; Rudolf, J. D.; Xie, P.; Xie, G.; Teng, Q.; Lohman, J. R.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Duan, Y.; Shen, B. (2014) Strain prioritization for natural product discovery by a high-throughput real-time PCR method. J. Nat. Prod. 77:2296-2303. [Pubmed]

189. Koch, A.; Gawron, D.; Steyaert, S.; Ndah, E.; Crappé, J.; De Keulenaer, S.; De Meester, E.; Ma, M.; Shen, B.; Gevaert, K.; Van Criekinge, W.; Van Damme, P.; Menschaert, G. (2014) A proteogenomics approach integrating proteomics and ribosome profiling increases the efficiency of protein identification and enables the discovery of alternative translation start sites. Proteomics 14:2688-2698. [Pubmed]

188. Ge, H.-M.; Huang, T.; Rudolf, J. D.; Lohman, J. R.; Huang, S.-X.; Guo, X.; Shen, B. (2014) Enediyne polyketide synthases stereoselectively reduce the ß-ketoacyl intermediates to ß-D-hydroxyacyl intermediates in enediyne core biosynthesis. Org. Lett. 16:3958-3961. [Pubmed]

187. Beerman, T. A.; Gawron, L. S.; Shen, B.; Kennedy, D. R. (2014) The radiomimetic enediyne, 20′-deschloro-C-1027 induces inter-strand DNA crosslinks in hypoxic cells and overcomes cytotoxic radioresistance. DNA Repair 21:165-170. [Pubmed]

186. Yin, M.; Yan, Y.; Lohman, J. R.; Huang, S.-X.; Ma, M.; Zhao, G.-R.; Xu, L.-H.; Xiang, W.; Shen, B. (2014) Cycloheximide and actiphenol production in Streptomyces sp. YIM56141 governed by single biosynthetic machinery featuring an acyltransferase-less type I polyketide synthase. Org. Lett. 16:3072-3075. [PubMed]

185. Xie, P.; Ma, M.; Rateb, M. E.; Shaaban, K. A.; Yu, Z.; Huang, S.-X.; Zhao, L.-X.; Zhu, X.; Yan, Y.; Peterson, R. M.; Lohman, J. R.; Yang, D.; Yin, M.; Rudolf, J. D.; Jiang, Y.; Duan, Y.; Shen, B. (2014) Biosynthetic potential-based strain prioritization for natural product discovery – a showdase for diterpenoid producing actinomycetes. J. Nat. Prod. 77:377-387. [PubMed]

184. Peterson, R. M.; Huang, T.; Rudolf, J. D.; Smanski, M. J.; Shen, B. (2014) Mechanisms of self-resistance in the platensimycin and platencin producing Streptomyces platensis MA7327 and MA7339 strains. Chem. Biol. 21:389-397. [PubMed]

183. Lohman, J. R.; Ma, M.; Cuff, M. E.;  Bigelow, L.; Bearden, J.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Sheb, B. (2014) The crystal structure of BlmI as a model for nonribosomal peptide synthetase peptidyl carrier proteins. Proteins, 82:1210-1218. PDF

182. Rateb, M. E.; Yu, Z.; Yan, Y.; Yang, D.; Huang, T.; Vodanovic-Jankovic, S.; Kron, M. A.; Shen, B. (2014) Medium optimization of Streptomyces sp. 17944 for tirandamycin B production and isolation and structural elucidation of tirandamycins H, I, and J. J. Antibiot. 67:127-132. [PubMed]

2013 Publications

181. Falzone, M.; Martens, E.; Tynan, H.; Maggio, C.; Golden, S.; Nayda, V.; Crespo, E.; Inamine, G.; Gelber, M.; Lemense, R.; Chiapinni, N.; Friedman, E.; Shen, B.; Gullo, V.; Demain, A. L. (2013) Development of a chemically-defined medium for the production of the antibiotic platensimycin by Streptomyces platensis. Appl. Microbiol. Biotechnol.97:9535-9539. [PubMed]

180.  Horsman, G. P.; Lechner, A.; Ohnishi, Y, Moore, B. S.; Shen, B. (2013) Predictive model for epoxide hydrolase-generated stereochemistry in the biosynthesis of 9-membered enediyne antitumor antibiotics. Biochemistry 52:5217-5224. PMID: 23844627 PDF

179.  Huang, S.-X.; Lohman, J. R.; Huang, T.; Shen, B. (2013) A new member of the 4-methylideneimidazole-5-one-containing aminomutase family from the enediyne kedarcidin biosynthetic pathway.  Proc. Natl. Acad. Sci. USA 110:8069-8074. PMID: 23633564 PDF  

178.  Ma, M.; Kwong, T.; Lim, S.-K.; Lohman, J. R.; Shen, B. (2013) The post-polyketide synthase steps in iso-migrastatin biosynthesis featuring tailoring enzymes with broad substrate specificity.  J. Am. Chem. Soc. 135:2489-2492. PDF

177.  Lohman, J. R.; Huang, S.-X.; Horsman, G.P.; Dilfer, P.; Huang, T.; Chen, Y.; Wendt-Pienkowski, E.; Shen, B. (2013) Cloning and sequencing of the kedarcidin biosynthetic gene cluster from Streptoalloteichus sp. ATCC 53650 revealing new insights into biosynthesis of the enediyne family of antitumor antibiotics.  Mol. BioSyst. 9:478-91. PMID: 23360970 PDF

176.  Lohman, J. R,; Bingman, C. A.; Phillips Jr., G. N.; Shen, B. (2013) The structure of the bifunctional acyltransferase/decarboxylase LnmK from the leinamycin biosynthetic pathway revealing novel activity for a double hot dog fold.  Biochemistry 52:902-911. [PubMed] PDF

175.  Yu, Z.; Rateb, M. E.; Smanski, M. J.; Peterson, R. M.; Shen, B. (2013) Isolation and structural elucidation of glucoside congeners of platencinfrom Streptomyces platensis SB12600. J. Antibiot. 66:291-294. PMID: 23361357 PDF

174.  Van der Donk, et al. (total of 58 co-authors). (2013) Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature. Nat. Prod. Rep. 30:108-160. PMID: 23165928 PDF

173.  Kurata, S.; Shen, B.; Liu, J. O.; Takeuchi, N.; Kaji, A.; Kaji, H. (2013) Possible steps of complete disassembly of post-termination complex by yeast eEF3 deduced from inhibition by translocation inhibitors. Nucleic Acids Res. 41:264-276. [PubMed] PDF

172.  Unsin, C. E.-M.; Rajski, S. R.; Shen, B. (2013) “The Role of Genetic Engineering in Natural Product-based Anticancer Drug Discovery” in Natural Products and Cancer Drug Discovery, Koehn, F. E. ed., Springer, New York, NY, pp175-191. PDF

171.  Tebbets, B.; Yu, Z.; Stewart, D.; Zhao, L.-X.; Jiang, Y.; Xu, L.-H.; Shen, B.; Klein, B. (2013) Identification of macrotetrolides as antifungal natural products via yeast bioassay: insights into drug spectrum, potency and mode of action.  Medical Mycology, 51:280-9. [PubMed]

2012 Publications

170.  Smanski, M. J.; Casper, J.; Peterson, R. M.; Yu, Z.; Rajski, S. R.; Shen B. (2012) Heterologous expression of the platencin gene cluster yields several platencin congeners.  J. Nat. Prod. 75:2158-2167. [PubMed] PDF

169.  Stern-Ginossar, N.; Weisburd, B.; Michalski, A.; Le, V. T. K.; Hein, M. Y.; Huang, S.-X.; Ma, M.; Shen, B.; Qian, S.-B.; Hengel, H.; Mann, M.; Ingolia, N. T.; Weissman, J. S. (2012) Decoding human cytomegalovirus.  Science 338:1088-1093. PMID: 23180859 PDF

168.  Yu, Z.; Vodanovic-Jankovic, S.; Kron, M.; Shen, B. (2012) New WS9326A Congeners from Streptomyces sp. 9078 inhibiting Brugia malayi asparaginyl-tRNA synthetase.  Org. Lett. 14:4946-4949. [PubMed] PDF

167.  Lee, S.; Liu, B.; Lee, S.; Huang, S.-X.; Shen, B.; and Qian, S.-B. (2012) Global mapping of translation initiation sites in mammalian cells at single-nucleotide resolution. Proc. Natl. Acad. Sci. USA 109:14728-14729 (E2424-E2432). [PubMed] PDF 

166.  Huang, S.-X.; Feng, Z.; Wang, L.; Galm, U.; Wendt-Pienkowski, E.; Yang, D.; Tao, M.; Coughlin, J. M.; Duan, Y.; Shen, B. (2012) A designer bleomycin with significantly improved DNA cleavage activity.  J. Am. Chem. Soc. 134:13501-13509. [PubMed] PDF

165.  Smanski, M. J.; Qu, X.; Liu, W., Shen, B. (2012) “Biosynthesis of pharmaceutical natural products and their pathway engineering”  Chapter 5 In “Organic Chemistry – Breakthroughs and Perspectives”, Ding, K.; Dai, L. eds., Wiley VCH, pp125-180.

164.  Lin, S.; Huang, T.; Shen, B. (2012) Tailoring enzymes acting on carrier protein-tethered substrates in natural product biosynthesis. Methods Enzymol. 516:321-343. [PubMed] PDF

163.  Lohman, J. R.;  Shen, B. (2012) 4-Methylideneimidazole-5-one-containing aminomutases in enediyne biosynthesis. Methods Enzymol. 516:299-319. [PubMed] PDF

162.  Smanski, M. J.; Peterson, R. M.;  Shen, B. (2012) Chapter 8: Platensimycin and platencin biosynthesis in Streptomyces platensis showcasing discovery and characterization of novel diterpene synthases. Methods Enzymol.515:163-186. [PubMed] PDF

161.  Lin, S.; Huang, T.; Horsman, G. P.; Huang, S.-X.; Guo, X.; Shen, B. (2012) Specificity of the ester bond-forming condensation enzyme SgcC5 in C-1027 biosynthesis. Org. Lett. 14:2300-2303.[PubMed]  PDF

160.  Smanski, M. J.; Peterson, R. M.; Huang, S.-X.; Shen, B. (2012) Bacterial diterpene synthases: new opportunities for mechanistic enzymology and engineered biosynthesis. Curr. Opinion Chem. Biol. 16:132-141. [PubMed] PDF

159.  Shen, B.; Thorson, J. S. (2012) Expanding nature’s chemical repertoire through metabolic engineering and biocatalysis. Curr. Opinion Chem. Biol. 16:99-100. [PubMed] PDF

158.  Yang, D.; Li, W.; Huang, S.-X.; Shen, B. (2012) Functional characterization of ttnI completing the tailoring steps for tautomycetin biosynthesis in Streptomyces griseochromogenes. Org. Lett. 14:1302-1305. [PubMed] PDF

157.  Huang, S.-X.; Wang, X.-J.; Yan, Y.; Wang, J.-D.; Zhang, J.; Liu, C.-X.; Xiang, W.-S.; Shen, B. (2012) Neaumycin – a new macrolide from Streptomyces sp. NEAU-x211. Org Lett. 14:1254-1257. [Pubmed]

156.  Van Lanen, S.G.; Shen, B. (2012) “Combinatorial Biosynthesis of Anticancer Natural Products” in Antitumor Agents from Natural Products, 2^nd Ed., Kingston, D.G.I.; Cragg, G.M.; Newman, D.J. eds., CRC Press Boca Raton, pp667-694.

2011 Publications

155.  Zhao, L.-X.; Huang, S.-X.; Tang, S.-K.; Jiang, C.-L.; Duan, Y.; Beutler, J. A.; Henrich, C. J.; McMahon, J. B.; Schmid, T.; Blees, J. S.; Colburn, N. H.; Rajski, S. R.; Shen, B. (2001) Actinopolysporins A-C and tubercidin as a Pdcd4 stabilizer from the halophilic actinomycete Actinopolyspora erythraea YIM 90600. J. Nat. Prod. 74:1990-1995. [PubMed]

154.  Smanski, M. J.; Yu, Z.; Casper, J.; Lin, S.; Peterson, R. M.; Chen, Y.; Wendt-Pienkowski, E.; Rajski, S. R.; Shen, B. (2011) Dedicated ent-Kaurene and ent-Atiserene Synthases for Platensimycin and Platencin Biosynthesis. Proc. Natl. Acad. Sci. USA 108:13498-13503. [PubMed]

153.  Yin, M.; Lu, T.; Zhao, L.-X.; Chen, Y.; Huang, S.-X.; Lohman, J. R.; Xu, L.-H.; Jiang, C.-L.; Shen, B. (2011) The missing C-17 O-methyltransferase in geldanamycin biosynthesis. Org. Lett. 13:3726-3729. [PubMed]

152.  Yu, Z.; Vodanovic-Jankovic, S.; Ledeboer, N.; Huang, S.-X.; Rajski, S. R.; Kron, M.; Shen, B. (2011) Tirandamycins from Streptomyces sp. 17944 Inhibiting the Parasite Brugia malayi Asparagin tRNA Synthetase. Org. Lett. 13:2034-2037. [PubMed]

151.  Chen, Y.; Yin, M.; Horsman, G. P.; Shen, B. (2011) Improvement of the enediyne antitumor antibiotic C-1027 production by manipulating its biosynthetic pathway regulation in Streptomyces globisporus. J. Nat. Prod. 74:420-424. [PubMed]

150.  Galm, U.; Wendt-Pienkowski, E.; Wang, L.; Huang, S.; Unsin, C.; Tao, M.; Coughlin, J. M.; Shen, B. (2011) Comparative analysis of the biosynthetic gene cluster and pathways for three structurall related antitumor antibiotics bleomycin, tallysomycin and zorbamycin. J. Nat. Prod. 74:526-536. [PubMed] PDF

149.  Yang, D.; Zhu, X.; Wu, X.; Feng, Z.; Huang, L.; Shen, B.; Zhang, Y. (2011) Titer improvement of iso-migrastatin in the selected heterologous Streptomyces hosts and related analysis of mRNA expression by quantitative RT-PCR. Appl. Microbiol. Biotechnol. 89:1709-1719. [PubMed]

148.  Zhang, Q.; Li, Y.; Chen, D.; Yu, Y.; Duan, L.; Shen, B.; Liu, W. (2011) Radical-mediated enzymatic carbon chain fragmentation-recombination. Nat. Chem. Biol. 7:154-160. [PubMed]

147.  Liu, S.; Yu, Z.; Yu, X.; Huang, S.-X., Luo, Y.; Wu, L.; Yang, Z.; Wang, L.; Gunawan, A.; Chan, R.J.; Shen, B.; Zhang, Z.-Y. (2011) SHP2 is a target of the immunosuppressant tautomycetin. Chem. Biol. 18:101-110. [PudMed]

146.  Huang, S.-X.; Yu, Z.; Robert, F., Zhao, L.-X.; Jiang, Y.; Duan, Y.; Pelletier, J., Shen, B. (2011) Cycloheximide and congeners as inhibitors of eukaryotic protein synthesis from endophytic actinomycetes Streptomyces sps. YIM56132 and YIM56141. J. Antibiot. 64:164-166. [PubMed]

145.  Yu, Z.; Zhao, L.-X.; Jiang, C.-L.; Duan, Y.; Wong, L.; Carver, K. C.; Schuler, L. A.; Shen, B. (2011) Bafilomycins produced by an endophytic actinomycete Streptomyces sp. YIM56209. J. Antibiot. 64:159-162. [PubMed]

144.  Shen, B. (2011) Editorial for the special issue of The Journal of Antibiotics dedicated to the late Professor C Richard Hutchinson. J. Antibiot. 64:3-5.

143.   Huang, Y.; Huang, S.-X.; Ju, J.; Tang, G.; Liu, T.; Shen, B. (2011) Functional characterization of the lnmKLM genes unveiling key intermediates for β-alkylation in leinamycin biosynthesis. Org. Lett. 13:498-501.[PubMed]

2010 Publications

142.  Chen, Y.; Wendt-Pienkowski, E.; Ju, J.; Rajski, S.R.; Shen, B. (2010) Characterization of FdmV as an amide synthetase for fredericamycin A biosynthesis in Streptomyces griseus ATCC43944. J. Biol. Chem. 285:38853-38860. [PubMed] 

141.  Yu, Y.; Guo, H.; Zhang, Q.; Duan, L., Ding, Y.; Lei, C.; Shen, B.; Liu, W. (2010) NosA catalyzing carboxyl-terminal amide formation in nosiheptide maturation via an enamine dealkylation on the serine-extended precursor peptide. J. Am. Chem. Soc. 132:16324-16326. [PubMed]

140.  Wu, X.; Yang, D.; Zhu, X.; Feng, Z.; Lv, Z.; Zhang, Y.; Shen, B.; Xu, Z. (2010) iso-Migrastatin titer improvement in the engineered Streptomyces lividans SB11002 strain by optimization of fermentation conditions. Biotechnol Bioprocess Eng. 15:664-669. [PubMed]

139.  Rajski, S. R.; Shen, B. (2010) Multifaceted modes of action for the glutarimide-containing polyketides revealed. ChemBioChem 11:1951-1954. [PubMed]

138.  Lin, S.; Horsman, G.P.; Shen, B. (2010) Characterization of the epoxide hydrolase NcsF2 from the neocarzinostatin biosynthetic gene cluster. Org. Lett. 12:3816-3819. [PubMed] PDF

137.  Ling, J.; Horsman, G. P.; Huang, S.-X.; Luo, Y.; Lin, S.; Shen, B. (2010) Enediyne antitumor antibiotic maduropeptin biosynthesis featuring a C-methyltransferase that acts on a CoA-tethered aromatic substrate. J. Am. Chem. Sc.132:12534-12536. [PubMed]

136.  Powell, E.; Huang, S.-X.; Xu, Y.; Rajski, S.R.; Wang, Y.; Peters, N.; Guo, S.; Xu, H.E.; Hoffmann, F.M.; Shen, B.; Xu, W. (2010) Identification and characterization of a novel estrogenic ligand actinopolymorphol A. Biochem. Pharmacol.80:1221-1229. [PubMed]

135.  Huang, S.-X.; Powell, E.; Rajski, S.R.; Zhao, L.; Jiang, C.; Duan, Y.; Xu, W.; Shen, B.  (2010) Discovery and total Synthesis of a new estrogen receptor heterodimering actinopolymorphol A from Actinopolymorpha rutilus.  Org. Lett.12:3525-3527. [PubMed]

134.  Chen, Y.; Yin, M.; Horsman, G. P.; Huang, S.;  Shen, B. (2010)  Manipulation of pathway regulation in Streptomyces globisporus for overproducing the enediyne antitumor antibiotic C-1027. J. Antibiot. 63:482-485. [PubMed]

133.  Horsman, G. P.; Chen, Y.; Thorson, J. S.; Shen, B. (2010)  Polyketide synthase chemistry does not direct biosynthetic divergence between 9- and 10-membered enediynes. Proc. Natl. Acad. Sci. U.S.A. 107:11331-11335.  PDF

132.  Zhao, C.; Coughlin, J. M.; Ju, J.; Zhu, D.; Wendt-Pienkowski, E.; Zhou, X.; Wang, Z.; Shen, B.; Deng, Z. (2010) Oxazolomycin biosynthesis in Streptomyces albus JA3453 featuring an “acyltransferase-less” polyketide synthase that incorporates two distinct extender units. J. Biol. Chem. 285:20097-20108. [PubMed]

131.  Luo, Y.; Li, W.; Ju, J.; Yuan Q.; Peters, N. R.; Hoffmann, F. M.; Huang, S.; Bugni, T.; Rajski, S. R.; Osada, H.; Shen, B. (2010) Functional characterization of TtnD and TtnF unveiling new insights into tautomycetin biosynthesis. J. Am. Soc. Chem. 132:6663-6671. [PubMed]

130.  Yu, Z.; Smanski, M. J.; Peterson, R. M.; Marchillo, K.; Andes, D.; Rajski, S. R.; Shen, B. (2010) Engineering of Streptomyces platensis MA7339 for overproduction of platencin and congeners. Org. Lett. 12:1744-1747. [PubMed]

129.  Zhang, N.; Zhu, X.; Yang, D.; Cai, J.; Tao, M.; Wang, L.; Duan, Y.; Shen, B.; Xu, Z. (2010) Improved production of tallysomycin H-1 in Streptomyces hindustanus SB8005 strain by fermentation optimization. Appl. Microbiol. Biotechnol.86:1345-1353. [PubMed]

128.  Chen, Y.; Smanski, M. J.; Shen, B. (2010) Improvement of secondary metabolite production in Streptomyces by manipulating pathway regulation. Appl. Microbiol. Biotechnol. 86:19-25. [PubMed]

127.  Schneider-Poetsch, T.; Ju, J.; Eyler, D. E.; Dang, Y.; Bhat, S.; Merrick, W. C.; Green, R.; Shen, B.; Liu, J. O. (2010) Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin. Nat. Chem. Biol. 6:209-217. [PubMed]

126.  Tao, M.; Wang, L.; Wendt-Pienkowski, E.; Zhang, N.; Yang, D.; Galm, U.; Coughlin, J. M.; Xu, Z.; Shen, B. (2010) Functional characterization of tlmH in Streptoalloteichus hindustanus E465-94 ATCC31158 unveiling new insight into tallysomycin biosynthesis and affording a new bleomycin analog. Mol. BioSyst. 6:349-356. [PubMed]

2009 Publications

125.  Lin, S.; Horsman, G. P.; Chen, Y.; Li, W.; Shen, B. (2009) Characterization of the SgcF epoxide hydrolase supporting a (R)-vicinal diol intermediate for enediyne antitumor antibiotic C-1027 biosynthesis. J. Am. Chem. Soc. 131:16410-16417. [PubMed]

124.  Cooke, H. A.; Guenther, E. L.; Luo, Y.; Shen, B.; Bruner, S. G. (2009) Molecular basis of substrate promiscuity for the SAM-dependent NcsB1 O-methyltransferase involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin� Biochemistry 48:9590-9598. [PubMed]  PDF

123.  Lim, S.-K.; Ju, J.; Zazopoulos, E.; Jiang, H.; Seo, J.-W.; Chen, Y.; Feng, Z.; Rajski, S. R.; Farnet, C. M.; Shen, B. (2009) iso-Migrastatin, migrastatin, and dorrigocin production in Streptomyces platensis NRRL18993 is governed by a single biosynthetic machinery featuring an acyltransferase-less type I polyketide synthase. J. Biol. Chem. 284:29746-29756. [PubMed]

122.  Van Lanen, S. G.; Lin, S.; Horsman, G. P.; Shen, B. (2009) Characterization of SgcE6, the flavin reductase component supporting FAD-dependent halogenation and hydroxylation in the biosynthesis of the enediyne antitumor antibiotic C-1027. FEMS Microbiol Lett. 300:237-241. [PubMed]

121.  Yu, Y.; Duan, L.; Zhang, Q.; Liao, R.; Ding, Y.; Pan, H.; Wendt-Pienkowski, E.; Tang, G.; Shen, B.; Liu, W. (2009) Nosiheptide biosynthesis featuring a unique indole side ring formation on the characteristic thiopeptide framework. ACS Chem. Biol. 4:855-864. [PubMed]

120.  Chen, Y.; Wendt-Pienkowski, E.; Rajski, S. R.; Shen, B. (2009) In vivo investigation of the roles of FdmM and FdmM1 in fredericamycin biosynthesis unveiling a new family of oxygenases. J. Biol. Chem. 284:24735-24743. [PubMed]

119.  Pinchot, S. N.; Adler, J. T.; Luo, Y.; Ju, J.; Li, W.; Shen, B.; Kunnimalaiyaan, M.; Chen, H. (2009) Tautomycin suppress the growth and neuroendocrine hormone markers in carcinoid cells through activation of the raf-1 pathway. Am. J. Surg. 197:313-319. [PubMed]

118.  Liu, T.; Huang, Y.; Shen, B. (2009) The bifunctional acyltransferase/decarboxylase LnmK as the missing link for β-alkylation in polyketide biosynthesis. J. Am. Chem. Soc. 131:6900-6901. [PubMed]

117.  Adler, J.; Cook, M.; Luo, Y.; Pitt, S.; Ju, J.; Li, W.; Shen, B.; Kunnimalaiyaan, M.; Chen, H. (2009) Tautomycetin and tautomycin suppress the growth of medullary thyroid cancer cells via inhibition of GSK-3beta. Mol. Cancer Therap. 8:914-920. [PubMed]

116.  Ju, J.; Li, W.; Yuan, Q.; Peters, N. ; Hoffmann, F.M., Rajski, S.R.; Osada, H.; Shen, B. (2009) Functional characterization of ttmM unveils new tautomycin analogs and insight into tautomycin biosynthesis and activity. Org. Lett. 11:1639-1642. [PubMed]

115.  Huang, S.-X.; Zhao, L.-X.; Tang, S.-K.; Jiang, C.-L.; Duan, Y.; Shen, B. (2009) Erythronolides H and I, new erythromycin congeners from a halophilic actinomycete Actinopolyspora sp. YIM90600. Org. Lett. 11:1353-1356. [PubMed]

114.  Wang, L.; Tao, M.; Wendt-Pienkowski, E.; Galm, U.; Coughlin, J.M.; Shen, B. (2009) Functional characterization of tlmK unveiling unstable carbinolamide intermediates in the tallysomycin biosynthetic pathway. J. Biol. Chem. 284:8256-8264. [PubMed]

113.  Liao, R.; Duan, L.; Lei, C.; Pan, H.; Ding, Y.; Zhang, Q.; Chen, D.; Shen, B.; Yu, Y.; Liu, W. (2009) Thiopeptide biosynthesis featuring ribosomally synthesized precursor peptides and conserved posttranslational modifications. Chem. Biol. 16:141-147. [PubMed]

112.  Li, W.; Luo, Y.; Ju, J.; Rajski, S.R. ; Osada, H. ; Shen, B. (2009) Characterization of the tautomycetin biosynthetic gene cluster from Streptomyces griseochromogenes provides new insight into dialkylmaleic anhydride biosynthesis. J. Nat. Prod. 72:450-459. [PubMed]

111.  Smanski, M.J.; Peterson, R.M.; Rajski, S.R.; Shen, B. (2009) Engineered Streptomyces platensis strains that overproduce antibiotics platensimycin and platencin. Antimicrob. Agents Chermother. 53:1299-1304. [PubMed]

110.  Cheng, Y.-Q.; Coughlin, J.M.; Lim, S.-K.; Shen, B. (2009) Type I polyketide synthases that require discrete acyltransferases. Methods in Enzymology 459:165-186. [PubMed]

109.  Jiang, H.; Rajski, S. R.; Shen, B. (2009) Tandem acyl carrier protein domains in polyunsaturated fatty acid synthases. Methods in Enzymology 459:79-96. [PubMed]

108.  Horsman, G.P.; Van Lanen, S.G.; Shen, B. (2009)  Iterative type I polyketide synthase for enediyne core biosynthesis. Methods in Enzymology 459:97-112. [PubMed]

107.  Rajski, S.R.; Shen, B. (2009) The role of evolution in the discovery of new drugs and chemicals. In ACS Symposium Series 1025: Chemical Evolution II: From Origins of Life to Modern Society, Zaikowski, L.; Friedrich, J.M.; Seidel, S.R. Eds., ACS, Washington DC, pp205-232.

106.  Lin, S.; Van Lanen, S.G.; Shen, B. (2009) A free-standing condensation enzyme catalyzing ester bond formation in C-1027 biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 106:4183-4188. [PubMed]

105.  Ju, J.; Rajski, S.R.; Lim, S.-K.; Seo, J.-W.; Peters, N.R.; Hoffmann, F.M.; Shen, B. (2009) Lactimidomycin, iso-migrastatin and related glutarimide-containing 12-membered macrolides are extremely potent inhibitors of cell migration. J. Am. Chem. Soc. 131:1370-1371. [PubMed]

104.  Feng, Z,; Wang, L.; Rajski, S.R.; Xu, Z.; Coeffet-LeGal, M,F.; Shen, B. (2009) Engineered production of iso-migrastatin in heterologous Streptomyces hosts. Bioorg. Med. Chem. 17:2147-2159. [PubMed]

103.  Beerman, T.A.; Gawron, L.S.; Shin, S.; Shen, B.; McHugh, M.M. (2009) C-1027, a radiomimetic enediyne anticancer drug, preferentially target hypoxic cells. Cancer Res. 69:593-598. [PubMed]  PDF

102.  Galm, U.; Wendt-Pienkowski, E.; Wang, L.; George, N.P.; Oh, T.-J.; Yi, F.; Tao, M.; Coughlin, J.M; Shen, B. (2009) The biosynthetic gene cluster of zorbamycin, a member of the bleomycin family of antitumor antibiotics, from Streptomyces flavoviridis ATCC21892. Mol. BioSyst. 4:77-90. [PubMed]

Hutchinson, C.R.; Shen, B. (2009) Pharmacognosy in Action US Academia: University of Wisconsin-Madison. In The American Society of Pharmacognosy 50 Years of Progress in Natural Products Research 1959-2009. Cragg, G.M.; Beutler, J.A.; Jones, W.P. eds., The American Society of Pharmacognosy, Omnipress, Madison, Wisconsin, 2009, pp140-142.

Shen, B. (2009) Expanding Horizons: Combinatorial Biosynthesis. In The American Society of Pharmacognosy 50 Years of Progress in Natural Products Research 1959-2009. Cragg, G.M.; Beutler, J.A.; Jones, W.P. eds., The American Society of Pharmacognosy, Omnipress, Madison, Wisconsin, 2009, pp248-250.

2008 Publications

101.  Galm, U.; Wang, L.; Wendt-Pienkowski, E.; Tao, M.; Coughlin, J. M.; Shen, B. (2008) In vivo manipulation of the bleomycin biosynthetic gene cluster in Streptomyces verticillus ATCC15003 revealing new insights into its biosynthetic pathway. J. Biol. Chem. 283:28236-28245. PDF

100.  Li, W.; Ju, J.; Rajski, S. R. ; Osada, H. ; Shen, B. (2008) Characterization of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillatus unveiling new insights into dialkylmaleic anhydride and polyketide biosynthesis. J. Biol. Chem. 283:28607-28617.

99.  Ju, J.; Rajski, S. R.; Lim, S.-K. ; Seo, J.-W. ; Peters, N. ; Hoffmann, F. M.; Shen, B. (2008) Evaluation of new migrastatin and dorrigocin congeners unveils cell migration inhibitors with dramatically improved potency. Bioorg. Med. Chem. Lett. 18:5951-5954.

98.  Chen, Y.; Wendt-Pienkowski, E.; Shen, B. (2008) Identification and utilities of FdmR1 as a SARP activator for fredericamycin production in Streptomyces griseus ATCC49344 and heterologous hosts.  J. Bcateriol. 190:5587-5596. [PubMed]

97.  Jiang, H.; Zirkle, R.; Metz, J. G.; Braun, L.; Richter, L.; Van Lanen, S. G.; Shen, B. (2008) The role of tandem acyl carrier protein domains in polyunsaturated fatty acid biosynthesis.  J. Am. Chem. Soc. 130:6336-6337. (Featured in Nat. Chem. Biol. 2008, 4:340) [PubMed]

96.   Luo, Y.; Lin, S.; Zhang, J.; Cooke, H. A.; Bruner, S. D.; Shen, B. (2008)  Regiospecific O-methylation of naphthoic acids catalyzed by NcsB1, an O-methyltransferase involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin. J. Biol. Chem. 283:14694-14702. [PubMed] PDF

95.  Lin, S.; Van Lanen, S. G.; Shen, B. (2008) Characterization of the two-component, FAD-dependent monooxygenase SgcC that requires carrier protein-tethered substrates for the biosynthesis of the enediyne antitumor antibiotic C-1027.  J. Am. Chem. Soc. 130:6616-6623. [PubMed] PDF

94.  Van Lanen, S. G.; Shen, B. (2008) Advances in polyketide synthase structure and mechanism. Curr. Opinion Drug Discov. Develop. 11:186-195. PDF

93.  Van Lanen, S. G.; Shen, B. (2008) Biosynthesis of enediyne antitumor antibiotics. Curr. Topics Med. Chem. 8:448-459. [PubMed]

92.  Song, D.; Coughlin, J.; Ju, J.; Zhou, X.; Shen, B.; Zhao, C.; Deng, Z. (2008) Alternative method for site-directed mutagenesis of complex polyketide synthase in Streptomyces albus JA3453. Acta Biochim. Biophys. Sinica 40:319-326. [PubMed]

91.  Chen, Y.; Luo, Y.; Ju.; Wendt-Pienkowski, E.; Rajski, S. R.; Shen, B. (2008) Identification of fredericamycin E from Streptomyces griseus: insights into fredericamycin A biosynthesis highlighting carbaspirocycle formation.  J. Nat. Prod.71:431-437. [PubMed]

90.  Zhang, J.; Van Lanen, S. G.; Ju, J.; Liu, W.; Dorrestein, P. C.; Li, W.; Kelleher, N. L.; Shen, B. (2008) A phosphopantetheinylating polyketide synthase producing a linear polyene to initiate enediyne antitumor antibiotic biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 105:1460-1465. [PubMed]

89.  Van Lanen, S. G.; Lin, S.; Shen, B. (2008) The biosynthesis of the antitumor antibiotic C-1027 involves a new branching point in chorismate metabolism. Proc. Natl. Acad. Sci. U.S.A. 105:494-499. (Featured in C & E News, January 14, 2008, p36) [PubMed]

88.  Montavon, T. J.; Christianson, C. V.; Festin, G. M.; Shen, B.; Bruner, S. D. (2008) Design and characterization of mechanism-based inhibitors for the tyrosine aminomutase SgTAM. Bioorg. Med. Chem. Lett. 18:3099-3102. [PubMed]

2007 Publications

87.  Christianson, C.V.; Montavon, T.J.; Festin, G.M.; Cooke, H.A.; Shen, B.; Bruner, S.D. (2007) The mechanism of MIO-based aminomutase chemistry in β-tyrosine biosynthesis. J. Am. Chem. Soc. 129:15744-15745.

86.  Ju, J.; Seo, J.-W.; Her, Y.; Lim, S.-K.; Shen, B. (2007) New Lactimidomycin Congeners Shed Insight into Lactimidomycin Biosynthesis in Streptomyces amphibiosporus. Org. Lett. 9:5183-5186. [ACS]

85.  Galm, U.; Shen, B. (2007) Natural product drug discovery: the times have never been better. Chem. Biol. 14:1098-1104. [Chem. Biol.]

84.  Van Lanen, S.G.; Oh T.-J.; Liu, W.; Wendt-Pienkowski, E.; Shen, B. (2007) Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. J. Am. Chem. Soc. 129:13082-13094. [ACS]  PDF

83.  Kennedy, D. R. ; Ju J.; Shen, B.; Beerman, T. A. (2007) Single modifications of an enediyne chromophore can confer an ability to induce DNA double strand breaks, interstrand crosslinks or both with concominant changes in PIKK regulation of DNA damage response. Proc. Natl. Acad. Sci. U.S.A. 104:17632-17637. [PNAS]  PDF

82.  Lin, S.; Van Lanen, S.G.; Shen, B. (2007) Regiospecific chlorination of (S)-β-tyrosyl-S-carrier protein catalyzed by SgcC3 in the biosynthesis of the enediyne antitumor antibiotic C-1027. J. Am. Chem. Soc. 129:12432-12438.[ACS]

81.  Tang, G.-L.; Cheng, Y.-Q.; Shen, B. (2007) Chain initiation in the leinamycin-producing hybrid non-ribosomal peptide/polyketide synthase from Streptomyces atroolivaceus S-140: discrete, monofunctional adenylation enzyme and peptidyl carrier protein that directly load (D)-alanine. J. Biol. Chem. 282:20273-20282. [JBC]

80.  Cooke, H. A.; Zhang, J.; Griffin, M. A.; Nonaka, K.; Van Lanen, S. G.; Shen, B.; Bruner, S. D. (2007) Characterization of NcsB2 as a Promiscuous Naphthoic Acid/Coenzyme A Ligase Integral to the Biosynthesis of the Enediyne Antitumor Antibiotic Neocarzinostatin. J. Am. Chem. Soc. 129:7728-7729. [ACS]  PDF

79.  Christianson, C. V.; Montavon, T. J.; Van Lanen, S. G.; Shen, B.; Bruner, S. D. (2007) The structure of L-tyrosine 2,3-aminomutase from C-1027 enediyne antitumor antibiotic biosynthetic pathway.  Biochemistry 46:7025-7214. [Pubmed]

78.  Guan, C.; Ju, J.;  Borlee, B. R.; Williamson, L. L.; Shen, B.; Raffa, K. F.; Handelsman, J. (2007)  Signal Mimics Derived from a Metagenomic Analysis of Gypsy Moth Gut Microbiota. Appl. Environ. Microbiol. 73:3669-3676. [AEM]

77.  Wang, L.; Yun, B.-S.; George, N. P.; Wendt-Pienkowski, E.; Galm, U.; Oh, T.-J.; Coughlin, J. M.; Zhang, G.; Tao, M.; Shen, B. (2007) The Glycopeptide Antitumor Antibiotic Zorbamycin from Streptomyces flavoviridis ATCC 21892: Strain Improvement and Structure Elucidation. J. Nat. Prod. 70:402-406. [Pubmed]

76.  Shen, B.; Cheng, Y.-Q.; Christenson, S. D.; Jiang, H.; Ju, J.; Kwon, H.-J.; Lim, S.-K.; Liu, W.; Nonaka, K.; Seo, J.-W.; Smith, W. C.; Standage, S.; Tang, G.-L.; Van Lanen, S.; Zhang, J. (2007) Polyketide biosynthesis beyond the type I, II, and III polyketide synthase paradigms: a progress report. In ACS symposium series 955, Polyketides: Biosynthesis, Biological Activities, and Genetic Engineering.  Rimando, A.M.; Baerson, S.R. eds., ACS, pp154-166.

75.  Kennedy, D. R.; Gawron, L. S.; Ju, J.; Liu, W.; Shen, B.; Beerman, T. A.  (2007) Single chemical modifications of the enediyne core of C-1027, a radiomimetic antitumor drug, result in markedly varied cellular responses to DNA double strand breaks. Cancer Res, 67:773-781. [Pubmed] PDF

74.  Tao, M.; Wang, L.; Wendt-Pienkowski, E.; George, N. P.; Galm, U.; Zhang, G.; Coughlin, J. M.; Shen, B. (2007) The tallysomycin biosynthetic gene cluster from Streptoalloteichus hindustanus E465-94 ATCC 31158 unveiling new insights into the biosynthesis of the bleomycin family of antitumor antibiotics. Molecular BioSystems, 3:60-74. [Pubmed]

2006 Publications

73.  Ju, J.; Lim, S.-K.; Jiang, H.; Seo, J.-W.; Her, H. Shen, B. (2006) Thermolysis of iso-migrastatin and its congeners via [3,3]-sigmatropic rearrangement: a new route to the synthesis of migrastatin and its analogs. Org. Lett. 8:5865.

72.  Van Lanen, S.; Shen, B. (2006) Biosynthesis of enediyne antitumor antibiotics. (2006) Drug Discovery Today: Technol. 3: 2006, 3, 285-292. Dorrestein, P. C.; Van Lanen, S. G.; Li, W.; Zhao, C.; Deng, Z.; Shen, B.; Kelleher, N. L. (2006) The bifunctional glyceryl transferase/phosphatase OzmB beloning to the HAD superfamily that diverts 1,3-biophosphoglycerate into polyketide biosynthesis. J. Am. Chem. Soc. 128:10386-10387.

71.  Galm, U.; Shen, B. (2006) Expression of biosynthetic gene clusters in heterologous hosts for natural product production and combinatorial biosynthesis. Exp. Opin. Drug Discov. 1:409-437.

70.  Van Lanen S.G.; Lin S.; Dorrestein, P.C.; Kelleher, N.L.; Shen B. (2006) Substrate specificity of the adenylation enzyme SgcC1 involved in the biosynthesis of the enediyne antitumor antibiotic C-1027. J. Biol. Chem. 281:29633-29640. PDF

69.  Dorrestein, P. C.; Van Lanen, S. G.; Li, W.; Zhao, C.; Deng, Z.; Shen, B.; Kelleher, N. L. (2006) The Bifunctional Glyceryl Transferase/phosphatase OzmB Belonging to the HAD Superfamily that Diverts 1,3-biophosphoglycerate into Polyketide Biosynthesis.  J. Am. Chem. Soc. 128:10386-102387.

68.  Huang, Y.; Wendt-Pienkowski, E.; Shen, B. (2006) A dedicated phosphopantetheinyl transferase for the fredericamycin polyketide synthase from Streptomyces griseus. J Biol. Chem. 281, 29660-29668.

67.  Zhao, C.; Ju, J.; Christenson, S. D.; Smith, W. C.; Song, D.; Zhou, X.; Shen, B.; Deng, Z. (2006) Utilization of the methoxymalony-acyl carrier protein biosynthetic locus for the cloning of the oxazolomycin biosynthetic gene cluster from Streptomyces albus.  J. Bacteriol. 198:4141-4147.

66.  Li, W.; Ju, J.; Osada, H.; Shen, B. (2006) Utilization of the methoxymalony-acyl carrier protein biosynthetic locus for the cloning of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillus. J. Bacteriol. 198:4148-4152.

65.  Van Lanen, S.; Shen, B. (2006) Microbial genomics for the improvement of natural product discovery. Curr. Opinion Microbiol. 9:252-262. PDF

64.  Tang, G.-L.; Cheng, Y.-Q.; Shen, B. (2006) Polyketide chain skipping mechanism in the biosynthesis of the hybrid nonribosomal peptide-polyketide antitumor antibiotic leinamycin in Streptomyces atroolivaceus S-140. J. Nat. Prod. 69:387-393. 

2005 Publications

63.  Wendt-Pienkowski, E.; Huang, Y.; Zhang, J.; Li, B.; Jiang, H.; Kwon,  H.-J.; Hutchinson, C.R.; Shen, B. (2005) Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus. J. Am. Chem. Soc. 127:16442-16452.[PubMed]

62.  Ju, J.; Lin, S-K.; Jiang, H.; Seo, J.-Woo S., Shen, B. (2005)  Iso-Migrastatin congeners from Streptomyces platensis and generation of a glutarimide polyketide library featuring the dorrigocin, lactimidomycin, migrastatin, and NK30424 scaffolds. J. Am. Chem. Soc. 127:11930-11931. [PubMed]

61.  Van Lanen, S.; Dorrestein, P. C.; Christenson, S. D.; Liu, W.; Ju, J.; Kelleher, N. L.; Shen, B. (2005) Biosynthesis of the β-amino acid  moiety of the enediyne antitumor antibiotic C-1027 featuring β-aminoacyl-S-carrier protein intermediates. J. Am. Chem. Soc. 127:11594-11595. [PubMed] PDF

60.  Ju, J.; Lim, S.-K.; Jiang, H.; Shen, B. (2004) Migrastatin and Dorrigocins Are Shunt Metabolites of iso-Migrastatin. J. Am. Chem. Soc. 127:1622-1623. [PubMed]

59.  Liu, W.; Nonaka, K.; Nie, P.; Zhang, J.; Christenson, S.D.; Bae, J.; Van Lanen, S.G.; Zazopoulos, E.; Farnet, C.M.; Yang, C.F.; Shen, B. (2005) The Neocarzinostatin Biosynthetic Gene Cluster from Streptomyces carzinostaticusATCC15944 involving two iterative type I polyketide synthase. Chem. Biol. 12:293-302. [PubMed] PDF

58.  Shen, B.; Liu, W.; Nonaka, K. (2005) ¨Enediyne Natural Products: Biosynthesis and Prospect towards Engineering Novel Antitumor Agents〃 in Frontiers in Medicinal Chemistry, Atta-ur-Rahman Ed., Bentham Science Publishers, 2005, Vol. 2, pp357-369.

57.  Galm, U.; Hager, M.H.; Van Lanen, S.G.; Ju, H.; Thorson, J.S.; Shen, B. (2005) Antitumor Antibiotics: Bleomycin, Enediynes, and Mitomycin. Chem. Rev. 105:739-758. [PubMed] PDF

56.  Thomas, M.G.; Bixby, K.A.; Shen, B. (2005) ¨Combinatorial Biosynthesis of Antitcancer Natural Products〃 in Antitumor Agents from Natural Products, Kingston, D.G.I.; Cragg, G.M.; Newman, D.J. eds., CRC Press, Boca Raton,  2005, 519-551.

55.  Shen, B.; Chen, M.; Cheng, Y.-Q.; Du, L.; Edwards, D.J.; George, N.P.; Huang, Y.; Oh, T.; Sanchez, C.; Tang, G.-L.; Wendt-Pienkowski, E.; Yi, F. (2005) Prerequisites for Combinatorial Biosynthesis: Evolution of Hybrid NRPS-PKS Gene Clusters in Biocombinatorial Approaches for Drug Finding, in Ernst Schering Research Foundation Workshop, Springer-Verlag, Berlin, 2005, 51:107-126.

2004 Publications

54.  Ju, J.; Ozanick, S. G.; Shen, B.; Thomas, M. G. (2004) Conversion of (2S)-Arginie to (2S, 3R)-Capreomycidine by VioC and VioD from the Viomycin Biosynthetic Pathway of Streptomyces sp ATCC11861. ChemBioChem, 5:1281-1285. [PubMed]

53.  Shen, B. (2004) Accessing natural products by combinatorial biosynthesis, Sci. STKE, Vol. 2004, Issue 225, pp. pe14. [PubMed]

52.  Murrell, J. M.; Liu, W.; Shen, B. (2004) Cloning and characterization of the SgcA1 a-D-glucopyranosyl-1-phosphate thymidylyltransferase from the enediyne antitumor antibiotic C-1027 biosynthetic pathway and overexpression of sgcA1 in Streptomyces globisporus to improve C-1027 production, J. Nat. Prod. 67:206-213. [PubMed] PDF

51.  Tang, G.-L.; Cheng, Y.-Q.; Shen, B. (2004) The Biosynthetic Gene Cluster of the Antitumor Antibiotic Leinamycin from Streptomyces atroolivaceus S-140 Revealing Unprecedented Architectural Complexity for a Hybrid Nonribosomal Peptide Synthetase-Polyketide Synthase.  Chem. Biol., 11:33-45. [PubMed]

2003 Publications

50.  Liu, W.; Ahlert, J.; Gao, Q.; Wendt-Pienkowski, E.; Shen, B.; Thorson, J.S. (2003) Rapid PCR Ampilification of Minimal Enediyne PKS Cassette Leads to a Predictive Familial Classification Model for Divergent Enediyne PKS Genes.  Proc. Natl. Acad. Sci. USA, 100:11959-11963.  [PubMed] PDF

49.  Christenson, S.D.; Wu, W.; Shen, B.; Toney, M.D. (2003) Kinetic Analysis of the 4-Methylideneimidazole-5-one-Containing Tyrosine Aminomutase in Enediyne Antitumor Antibiotic C-1027 Biosynthesis. Biochemistry, 42:12708-12718.  [PubMed]

48.  Schneider, T.L.; Shen, B.; Walsh, C.T. (2003) Oxidase domains in epothilone and bleomycin biosynthesis: thiazoline to thiazole oxidation during chain elongation. Biochemistry, 42:9722-9730.[PubMed] PDF

47.  Du, L.; Chen, M.; Zhang, Y.; Shen, B. (2003) The BlmIII and BlmIV nonribosomal peptide Synthetase-catalyzed biosynthesis of the bleomycin bithiazole moiety involving both in cis and in trans aminoacylation. Biochemistry, 42:9731-9740. [PubMed] PDF

46.  Shen, B.; Liu, W.; Nonaka, K. (2003) Enediyne Natural Products: Biosynthesis and Prospect towards Engineering Novel Antitumor Agents. Curr. Med. Chem. 10:2317-2325. [PubMed] PDF

45.  Christenson, S.D.; Liu, W.; Toney, M.D.; Shen, B. (2003) A novel dehydroalanine-dependent tyrosine aminomutase in C-1027 enediyne biosynthesis.  J. Am. Chem. Soc. 125:6062-6063. [PubMed]

44.  Shen, B. (2003) Polyketide biosynthesis beyond the type I, II, and III polyketide synthase paradigms.  Curr. Opinion Chem. Biol. 7:285-295. [PubMed] PDF

43.  Cheng, Y.-Q.; Tang, G.-L.; Shen, B. (2003) Type I polyketide synthase requiring a discrete acyltransferase for polyketide biosynthesis. Proc. Natl. Acad. Sci. USA, 100:3149-3154. [PubMed]

42. Du, L.; Cheng, Y.Q.; Ingenhorst, G.; Tang, G.-L.; Huang, Y.; Shen, B. (2003) Hybrid peptide-polyketide natural products: biosynthesis and prospects towards engineering novel molecules” in Genetic Engineering, Principle and Methods, Setlow, J. K. ed., Kluwer Academic/Plenum Publishers, vol 25, 227-267.

41.  Zazopoulos, E.; Huang, K.; Staffa, A; Liu, W.; Bachmann, B. O.; Nonaka, K.; Ahlert, J.; Thorson, J. T.; Shen, B.; Farnet, C. M. (2003) A genomics-guided approach for discovering and expressing cryptic metabolic pathways. Nat. Biotechnol. 21:187-190.[PubMed] PDF

2002 Publications

40.  Cheng, Y.-Q.; Tang, G.-L.; Shen, B. (2002) Identification and Localization of the Antitumor macrolactam Leinamycin Biosynthesis Gene Cluster from Streptomyces atroolivaceus S-140. J. Bacteriol., 184:7013-7024. [PubMed]

39.  Shen, B.; Kwon, H.-J. (2002) Macrotetrolide biosynthesis: a novel type II polyketide synthase, The Chemical Record, 2:389-396. [PubMed]

38.  Kwon, H.-J.; Smith, W. C.; Scharon, A. J.; Hwang, S. H.; Kurth, M. J.; Shen, B. (2002) C—O Bond Formation by Polyketide Synthases. Science, 297:1327-1330. [abstract] [Full text].

37.  Liu, W.; Christenson, S. D.; Standage, S.; Shen, B. (2002) Biosynthesis of the Enediyne Antitumor Antibiotic C-1027. Science, 297:1170-1173. [abstract] [Full text] PDF

36.  Shen, B.; Du, L.; Sanchez, C.; Chen, M.; Edwards, D.J.; Chen, M.; Murrell, J.M. (2002) Cloning and Characterization of the Bleomycin Biosynthetic Gene Cluster from Streptomyces verticillus ATCC15003. J. Nat. Prod. 65:422-431. [PubMed] PDF

2001 Publications

35.  Shen, B.; Du, L.; Sanchez, C.; Chen, M.; Edwards, D.J. (2001) A Model for Hybrid Peptide-Polyketide Biosynthesis: The Bleomycin Biosynthetic Gene Cluster. J. Ind. Microbiol. Biotechnol., 27:378-385. [PubMed] PDF

34.  Sanchez, C.; Du, L.; Edwards, D.J.; Toney, M.D,; Shen, B. (2001) Cloning and Characterization of a Phosphopantetheinyl Transferase from Streptomyces verticillus ATCC15003, the Producer of the Hybrid Peptide-Polyketide Antitumor Drug Bleomycin. Chem. Biol., 8:725-738. [PubMed] PDF

33.  Kwon, H.-J.; Smith, W.C.; Xiang, L.; Shen, B. (2001) Cloning and heterologous statement of the macrotetrolide biosynthetic gene cluster revealed a novel polyketide synthase that lacks an acyl carrier proteine. J. Am. Chem. Soc., 123:3385-3386. [PubMed]

32.  Rafanan Jr., E.R.; Le, L.; Zhao, L.; Decker, H.; Shen, B. (2001) Cloning, Sequencing, and Heterologous statement of the elmGHIJ Genes Involved in the Biosynthesis of the Polyketide Antibiotic Elloramycin from Streptomyces olivaceusTü2353. J. Nat. Prod., 64:444-449.  [PubMed]

31.  Shen, B.; Du, L. (2001) Biosynthesis of hybrid peptide-polyketide natural products. Curr. Opin. Drug Discov. Develop., 4:215-228. 

30.   Du, L.; Sanchez, C.; Shen, B. (2001) Biosynthesis of Hybrid Peptide and Polyketide Metabolites: Prospect towards Engineering Novel Molecules. Met. Engineer., 3:78-95. PDF 

2000 Publications

29.  Rafanan Jr., E.R.; Hutchinson, C.R.; Shen, B. (2000) Triple Hydroxylation of Tetracenomycin A2 to Tetracenomycin C Involving Two Molecules of O2 and One Molecule of H2O. Org. Lett., 2:3225-3227. [PubMed]

28.  Beynon, J.D.; Rafanan Jr., E.R.; Shen, B.; Fisher, A.J. (2000) Crystallization and Preliminary X-ray Analysis of Tetracenomycin A2 Oxygenase: A Flavoprotein Hydroxylase Involved in Polyketide Biosynthesis. Acta Cryst., D56:1647-1651. [PubMed]

27.  Du, L.; Chen, M.; Sanchez, C.; Shen, B. (2000) An Oxidation Domain in the BlmIII Nonribosomal Peptide Synthetase Probably Catalyzing Thiazole Formation in the Biosynthesis of the Antitumor Drug Bleomycin in Streptomyces verticillus ATCC15003. FEMS Microbiol. Lett., 189:171-175. [PubMed] PDF

26.  Du, L.; Sanchez, C.; Chen, M.; Edwards, D.J.; Shen, B. (2000) The Biosynthetic Gene Cluster for the Antitumor Drug Bleomycin from Streptomyces verticillus ATCC15003 Supporting Functional Interactions between Nonribosomal Peptide Synthetases and a Polyketide Synthase” Chem. Biol., 7:623-642. [PubMed] PDF

25.  Smith, W. C.; Xiang, L.; Shen, B. (2000) Genetic Localization and Molecular Characterization of the nonS Gene Required for Macrotetrolide Biosynthesis in Streptomyces griseus DSM40695. Antimicrob. Agents Chermother., 44:1809-1817. [PubMed]

24.  Liu, W.; Shen, B. (2000) Genes for Production of the Enediyne Antitumor Antibiotic C-1027 in Streptomyces globisporus Are Clustered with the cagA Gene That Encodes the C-1027 Apoprotein. Antimicrob. Agents Chermother., 44:382-392. [PubMed]

23.  Shen, B. (2000) Aromatic Polyketide Biosynthesis in Topics in Current Chemistry: Biosynthesis Vol. II, Leeper, F. J.; Vederas, J. C. eds, Springer-Verlag:Berlin, 209:1-51.

1999 Publications

22.  Du, L.; Shen, B. (1999) Identification and Characterization of a Type II Peptidyl Carrier Protein from the Bleomycin Producer Streptomyces verticillus ATCC15003. Chem. Biol., 6:507-517. [PubMed] PDF

21.  Thorson, J.S.; Shen, B.; Whitwam, R.E.; Liu, W.; Li, Y.; Ahlert, J. (1999) Enediyne Biosynthesis and Self-resistance: A Progress Report Bioorg. Chem., 27:172-188.

20.  Shen, B.; Du, L.; Sanchez, C.; Chen, M.; Edwards, D.J. (1999) Bleomycin Biosynthesis in Streptomyces verticillusATCC15003: A Model of Hybrid Peptide and Polyketide Biosynthesis” Bioorg. Chem., 27:155-171.  

1991-1996 Publications

19.  Shen, B.; Hutchinson, C. R. (1996) Deciphering the Mechanism for the Assembly of Aromatic Polyketides by a Bacterial Polyketide Synthase. Proc. Natl. Acad. Sci. U.S.A., 93:6600-6604. PDF

18.  Summers, R. G.; Ali, A.; Shen, B.; Wessel, W. L.; Hutchinson, C. R. (1995) Malonyl-Coenzyme A:Acyl Carrier Protein Acyltransferase of Streptomyces glaucescens: A Possible Link between Fatty Acid and Polyketide Biosynthesis. Biochemistry, 34:9389-9402. PDF

17.  Shen, B.; Summers, R. G.; Wendt-Pienkowski, E.; Hutchinson, C. R. (1995) The Streptomyces glaucescens tcmKLPolyketide Synthase and tcmN Polyketide Cyclase Genes Govern the Size and Shape of Aromatic Polyketides. J. Am . Chem. Soc., 117:6811-6821. PDF

16.  Shen, B.; Hutchinson, C. R. (1994) Triple-Hydroxylation of Tetracenomycin A2 to Tetracenomycin C in “Streptomyces glaucescens: Overexpression of the tcmG Gene in Streptomyces lividans and Characterization of the Tcm A2 Oxygenase. J. Biol. Chem., 48:30726-30733. PDF

15.  Shen, B.; Hutchinson, C. R. (1993) Tetracenomycin F2 Cyclase:  Intramolecular Aldol Condensation in the Biosynthesis of Tetracenomycin C in Streptomyces glaucescens. Biochemistry, 32:11149-11154. PDF

14.  Shen, B.; Hutchinson, C. R. (1993) Enzymatic Synthesis of a Bacterial Polyketide from Acetyl and Malonyl CoA by a Type II Polyketide Synthase. Science, 262:1535-1540. PDF

13.  Shen, B.; Hutchinson, C. R. (1993) Tetracenomycin F1 Monooxygenase:  Oxidation of a Naphthacenone to a Naphthacenequinone in the Biosynthesis of Tetracenomycin C in Streptomyces glaucescens.  Biochemistry, 32:6656-6663. PDF

12.  Shen, B.; Nakayama, H.; Hutchinson, C. R. (1993) Isolation and Structural Elucidation of Tetracenomycin F2 and Tetracenomycin Fl: Early Intermediates in the Biosynthesis of Tetracenomycin C in Streptomyces glaucescens.  J. Nat. Prod., 56:1288-1293. PDF

11.  Hutchinson, C. R.; Summers, R. G.; Shen, B.; Decker, H.; Wendt-Pienkowski, E.; Ali, A.; Grimm, A. C.; Suh, J. W.; Kwon, H. J.; and Wessel, W. L. (1993) Where Has the Manipulation of Bacterial Type II Polyketide Synthase Gene Led Us?  In Third International Conference on the Biotechnology of Microbial Products:  Novel Pharmacological and Agrobiological Activities, V. P. Gullo, J. C. Hunter-Cevera, R. Cooper, and R. K. Johnson, eds., SIM, Fredericksburg, Virginia, pp. 187-204.

10.  Hutchinson, C. R.; Decker, H.; Motamedi, H.; Shen, B.; Summers, R. G.; Wendt-Pienkowski, E.; Wessel, W. L. (1993) Molecular Genetics and Biochemistry of a Bacterial Type II Polyketide Synthase, in Industrial Microorganisms: Basic and Applied Molecular Genetics, R. H. Baltz, G. D. Hegeman, P. L. Skatrud, eds., ASM, Washington, D.C., pp. 203-216.

9.  Hutchinson, C. R.; Decker, H.; Guilfoile, P. G.; Wendt-Pienkowski, E.; Shen, B.; Summers, R. G. (1992) Molecular Biology of Antibiotic Production in Streptomyces glaucescens, in Harnessing Biotechnol. 21st Century, Proc. Int. Biotechnol. Symp. Expo. 9th, M. R. Ladisch, and A. Bose, eds., ACS, Washington, D.C., pp. 127-130.

8.  Hutchinson, C. R.; Decker, H.; Guilfoile, P. G.; Shen, B.; Summers, R. G.; Wendt-Pienkowski, E.; Wessel. W. L. (1992) Polyketide Synthase: Enzyme Complexes and Multifunctional Proteins Directing the Biosynthesis of Bacterial Metabolites from Fatty Acids, in Secondary Metabolite Biosynthesis and Metabolism, R. J. Petroski and S. P. McCormick, eds., Plenum Press, New York, pp. 3-10.

7.  Hutchinson, C. R.; Decker, H.; Motamedi, H.; Shen, B.; Summers, R. G.; Wendt-Pienkowski, R.; Wessel, W. L. (1992) Molecular Genetics and Biochemistry of Type II Polyketide Synthesis from the Perspective of Tetracenomycin C Biosynthesis in Streptomyces glaucescens. Actinomycetol., 6:49-68.

6.  Shen, B.; Summers, R. G.; Gramajo, H.; Bibb, M. J.; Hutchinson, C. R. (1992) Purification and Characterization of the Acyl Carrier Protein of the Streptomyces glaucescens Tetracenomycin C Polyketide Synthase. J. Bacteriol., 174:3818-3821. PDF

1986-1990 Publications

5.  Shen, B.; Gould, S. J. (1991) Opposite Facial Specificity for Two Hydroquinone Epoxidases: (3-si, 4-re)-2,5-Dihydroxyacetanilide Epoxidase from Streptomyces LL-C10037 and (3-re, 4-si)-2,5-Dihydroxyacetanilide Epoxidase from Streptomyces MPP 3051″. Biochemistry,30:8937-8944. PDF

4.  Gould, S. J.; Shen, B. (1991) Epoxyquinones from 2,5-Dihydroxyacetanilide: Opposite Facial Specificity in the Epoxydation by Enzymes from Streptomyces LL-C10037 and Streptomyces MPP 3051. J. Am. Chem. Soc., 113:684-686. PDF

3.  Shen, B.; Whittle, Y. G.;  Keszler, D. A.; Gould, S. J. (1990) Structure and Absolute Stereochemistry of the Epoxyquinol LL-C10037α and Related Metabolites from Streptomyces LL-C10037. J. Org. Chem., 55:4422-4426. 

2.  Gould, S. J.;  Shen, B.; Whittle, Y. G.  (1989) The Biosynthesis of LL-C10037α: The Steps beyond 3-Hydroxyanthranillic Acid. J. Am. Chem. Soc.111:7932-38. PDF

1.  Liu, C.; Chen, Q.; Yu, Z.; Shen, B. (1986) Mordenite-Catalyzed Three-Phase Hydration of α-Pinene: Product Distribution, Kinetics and Reaction Mechanism. Huaxue Xuebo, 44:545-50.